ArticlePlant cell reports2008
Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.The Journal of experimental biology · 2022Article
- Antifreeze protein from Ammopiptanthus nanus functions in temperature-stress through domain A.Scientific reports · 2021Article
- A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering.3 Biotech · 2019Review
- Advances in cryopreservation of organs.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2016Review
- Ectopic expression of Arabidopsis RCI2A gene contributes to cold tolerance in tomato.Transgenic research · 2015Article
- Overexpression of Ran gene from Lepidium latifolium L. (LlaRan) renders transgenic tobacco plants hypersensitive to cold stress.Molecular biology reports · 2014Article
- Isolation, molecular characterization, and functional analysis of the vacuolar Na+/H+ antiporter genes from the halophyte Karelinia caspica.Molecular biology reports · 2012Article
- Induced ectopic expression of At-CBF1 in marker-free transgenic tomatoes confers enhanced chilling tolerance.Plant cell reports · 2011Article
- The rearing and biology of the desert beetle, Microdera punctipennis, under laboratory conditions.Journal of insect science (Online) · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the function of antifreeze protein from Microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of MpAFP149 gene with the signal peptide sequence responsible for secreting the native MpAFP149 into the apoplast space under control of a cauliflower mosaic virus 35S promoter was introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. The observation of immunogold localization by TEM (transmission electron microscope) showed that the heterologous MpAFP149 protein was mainly distributed on the cell wall in apoplast of the transgenic tobacco plant. T1 generation transgenic tobacco plants displayed a more frost resistant phenotype and kept the lower ion leakage ratio and MDA (malondialdehyde) content in the leaves compared with wild-type ones at -1 degrees C for 3 days. The results showed that MpAFP149 provided protection and conferred cold tolerance to transgenic tobacco plant during freezing stress.
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