ArticleJournal of clinical microbiology2008
Sensitive detection of multiple rotavirus genotypes with a single reverse transcription-real-time quantitative PCR assay.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Systematic review of point-of-need molecular diagnostics for rotavirus and enteric adenoviruses F40/F41.BMC infectious diseases · 2026Pooled it
- Human enterovirus infections in children at increased risk for type 1 diabetes: the Babydiet study.Diabetologia · 2011Trial
- Complete Genome Sequencing of a G3P[14] Rabbit Rotavirus.Animals : an open access journal from MDPI · 2025Article
- Detection of canine distemper virus in wildlife in Italy (2022-2024).Frontiers in veterinary science · 2025Article
- Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus.Veterinary sciences · 2024Review
- Multisite community-scale monitoring of respiratory and enteric viruses in the effluent of a nursing home and in the inlet of the local wastewater treatment plant.Applied and environmental microbiology · 2024Article
- Genomic revelations: investigating rotavirus a presence in wild ruminants and its zoonotic potential.Frontiers in veterinary science · 2024Article
- Application of the cell-based RT-qPCR assay (C-QPA) for potency detection of the novel trivalent rotavirus vaccine in China.Journal of clinical laboratory analysis · 2023Article
- The impact and complete genome characterisation of viruses involved in outbreaks of gastroenteritis in a farrow-to-finish holding.Scientific reports · 2023Article
- Improved performance of nucleic acid-based assays for genetically diverse norovirus surveillance.Applied and environmental microbiology · 2023Article
- Rotavirus A in Domestic Pigs and Wild Boars: High Genetic Diversity and Interspecies Transmission.Viruses · 2022Article
- Genetic diversity of porcine reproductive and respiratory syndrome virus and evaluation of three one-step real-time RT-PCR assays in Korea.BMC veterinary research · 2022Article
- SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish.Environmental research · 2022Article
- Development of a Real-Time Reverse Transcription-PCR Assay To Detect and Quantify Group A Rotavirus Equine-Like G3 Strains.Journal of clinical microbiology · 2021Article
- Review
- A Remarkable Genetic Diversity of Rotavirus A Circulating in Red Fox Population in Croatia.Pathogens (Basel, Switzerland) · 2021Article
- Benign Convulsions with Mild Rotavirus and Norovirus Gastroenteritis: Nationwide Data from the Health Insurance Review and Assessment Service in South Korea.Children (Basel, Switzerland) · 2021Article
- Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission.Pathogens (Basel, Switzerland) · 2020Article
- The performance of biological and tertiary wastewater treatment procedures for rotaviruses A removal.Environmental science and pollution research international · 2020Article
- The emergence of porcine epidemic diarrhoea in Croatia: molecular characterization and serology.BMC veterinary research · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rotaviruses are one of the major causes of diarrhea in infants and children under 5 years old, especially affecting developing countries. In natural disasters, fecal matter and potable waters can mix, allowing low, yet infective, concentrations of rotavirus to be present in water supplies, constituting a risk for the population. Any of the most commonly detected rotavirus genotypes could originate an outbreak. The development of a fast and sensitive method that could detect the broadest possible range of rotavirus genotypes would help with efficient diagnosis and prevention. We have designed a reverse transcription (RT)-real-time quantitative PCR approach targeted to the rotaviral VP2 gene, based on a multiple-sequence alignment of different human rotaviral strains. To overcome the high nucleotide sequence diversity, multiple forward and reverse primers were used, in addition to a degenerate probe. The performance of the assay was tested on isolates representing the most prevalent human genotypes: G1P[8], G2P[4], G3P[8], G4P[8], G9P[8], and G12P[8]. The developed method improved classical rotavirus detection by enzyme-linked immunosorbent assay and nested RT-PCR by 5 and at least 1 order of magnitude, respectively. A survey of 159 stool samples indicated that the method can efficiently detect a broad range of rotavirus strains, including different G-P genotype combinations of human, porcine, and bovine origin. No cross-reactivity was observed with other enteric viruses, such as astrovirus, sapovirus, and norovirus.
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