ArticleJournal of clinical microbiology2008
Salmonella serovar identification using PCR-based detection of gene presence and absence.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Finding invisible quantitative trait loci with missing data.Plant biotechnology journal · 2018Article
- A simplified multiplex PCR-based typing method for commonThe Indian journal of medical research · 2017Article
- Effective characterization of Salmonella Enteritidis by most probable number (MPN) followed by multiplex polymerase chain reaction (PCR) methods.Environmental science and pollution research international · 2017Article
- PAV markers in Sorghum bicolour: genome pattern, affected genes and pathways, and genetic linkage map construction.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- The commercial impact of pig Salmonella spp. infections in border-free markets during an economic recession.Veterinary world · 2015Review
- Comparison of typing methods with a new procedure based on sequence characterization for Salmonella serovar prediction.Journal of clinical microbiology · 2013Article
- Variation of presence/absence genes among Arabidopsis populations.BMC evolutionary biology · 2012Article
- Automated pangenomic analysis in target selection for PCR detection and identification of bacteria by use of ssGeneFinder Webserver and its application to Salmonella enterica serovar Typhi.Journal of clinical microbiology · 2012Article
- Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives.Applied and environmental microbiology · 2011Review
- Article
- Spontaneous excision of the Salmonella enterica serovar Enteritidis-specific defective prophage-like element phiSE14.Journal of bacteriology · 2010Article
- Protection of Salmonella by ampicillin-resistant Escherichia coli in the presence of otherwise lethal drug concentrations.Proceedings. Biological sciences · 2009Article
- High-throughput molecular determination of salmonella enterica serovars by use of multiplex PCR and capillary electrophoresis analysis.Journal of clinical microbiology · 2009Article
- Novel genetic tools for studying food-borne Salmonella.Current opinion in biotechnology · 2009Article
- Rapid multiplex PCR and real-time TaqMan PCR assays for detection of Salmonella enterica and the highly virulent serovars Choleraesuis and Paratyphi C.Journal of clinical microbiology · 2008Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
There are more than 2,500 known Salmonella serovars, and some of these can be further subclassified into groups of strains that differ profoundly in their gene content. We refer to these groups of strains as "genovars." A compilation of comparative genomic hybridization data on 291 Salmonella isolates, including 250 S. enterica subspecies I strains from 32 serovars (52 genovars), was used to select a panel of 384 genes whose presence and absence among serovars and genovars was of potential taxonomic value. A subset of 146 genes was used for real-time PCR to successfully identify 12 serovars (16 genovars) in 24 S. enterica strains. A further subset of 64 genes was used to identify 8 serovars (9 genovars) in 12 multiplex PCR mixes on 11 S. enterica strains. These gene panels distinguish all tested S. enterica subspecies I serovars and their known genovars, almost all by two or more informative markers. Thus, a typing methodology based on these predictive genes would generally alert users if there is an error, an unexpected polymorphism, or a potential new genovar.
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