Evidence map›Paper›PMID 18524960›Full record

ArticleJournal of clinical microbiology2008

Real-time PCR assays to address genetic diversity among strains of Mycoplasma hyopneumoniae.

Erin L Strait, Melissa L Madsen, F Chris Minion, Jane Christopher-Hennings, Matthew Dammen, Katherine R Jones, Eileen L Thacker

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

  1. Article
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  7. Rapid detection ofFrontiers in cellular and infection microbiology · 2024
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  8. Hijacking of Host Plasminogen byMicrobiology spectrum · 2023
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  13. Seasonal Variation in Prevalence ofPathogens (Basel, Switzerland) · 2021
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  14. Article
  15. Article
  16. Diversity ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020
    Article
  17. Article
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Erin L StraitDepartment of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, USA.
Melissa L Madsen
F Chris Minion
Jane Christopher-Hennings
Matthew Dammen
Katherine R Jones
Eileen L Thacker

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasma hyopneumoniae is an important cause of pneumonia in pigs around the world, but confirming its presence in (or absence from) pigs can be difficult. Culture for diagnosis is impractical, and seroconversion is often delayed after natural infection, limiting the use of serology. Numerous PCR assays for the detection of M. hyopneumoniae have been developed, targeting several different genes. Recently, genetic diversity among strains of M. hyopneumoniae was demonstrated. The effect of this diversity on the accuracy and sensitivity of the M. hyopneumoniae PCR assays could result in false-negative results in current PCR tests. In this study, a panel of isolates of M. hyopneumoniae, M. flocculare, M. hyorhinis, and M. hyosynoviae were tested with a number of M. hyopneumoniae-specific PCR assays. Some M. hyopneumoniae PCR assays tested did not detect all isolates of M. hyopneumoniae. To increase the efficiency of PCR testing, two new real-time PCR assays that are specific and capable of detecting all of the M. hyopneumoniae isolates used in this study were developed.

Indexed as

Polymorphism, GeneticAnimalsDNA, BacterialDNA PrimersMolecular Sequence DataMycoplasma hyopneumoniaePneumonia of Swine, MycoplasmalPolymerase Chain ReactionSensitivity and SpecificitySequence AlignmentSequence Analysis, DNASwineDNA, BacterialDNA Primers

Identifiers

PMID18524960
PMCPMC2519509

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.