ArticleMolecular biology of the cell2008
Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
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- ARF GTPases and Their Ubiquitous Role in Intracellular Trafficking Beyond the Golgi.Frontiers in cell and developmental biology · 2021Review
- Endocytic Adaptor Proteins in Health and Disease: Lessons from Model Organisms and Human Mutations.Cells · 2019Review
- An emerging role for IQGAP1 in tight junction control.Small GTPases · 2018Review
- Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo.Scientific reports · 2017Article
- Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition.Molecular biology of the cell · 2017Article
- Article
- Protein tyrosine phosphatase PTPN3 inhibits lung cancer cell proliferation and migration by promoting EGFR endocytic degradation.Oncogene · 2015Article
- Endocytic adaptor epidermal growth factor receptor substrate 15 (Eps15) is involved in the trafficking of ubiquitinated α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors.The Journal of biological chemistry · 2014Article
- A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode.PloS one · 2013Article
- Molecular characterization of EGFR and EGFRvIII signaling networks in human glioblastoma tumor xenografts.Molecular & cellular proteomics : MCP · 2012Article
- A novel role for survivin in erythroblast enucleation.Haematologica · 2012Article
- The clathrin adaptor AP-1A mediates basolateral polarity.Developmental cell · 2012Article
- Basolateral sorting of the coxsackie and adenovirus receptor through interaction of a canonical YXXPhi motif with the clathrin adaptors AP-1A and AP-1B.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cholesterol-rich microdomains as docking platforms for respiratory syncytial virus in normal human bronchial epithelial cells.Journal of virology · 2012Article
- Sip1, a conserved AP-1 accessory protein, is important for Golgi/endosome trafficking in fission yeast.PloS one · 2012Article
- Hepatocytes internalize trophic receptors at large endocytic "Hot Spots".Hepatology (Baltimore, Md.) · 2011Article
- Recycling of the epidermal growth factor receptor is mediated by a novel form of the clathrin adaptor protein Eps15.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Eps15 (EGFR pathway substrate clone 15) is well known for its role in clathrin-coated vesicle formation at the plasma membrane through interactions with other clathrin adaptor proteins such as AP-2. Interestingly, we observed that in addition to its plasma membrane localization, Eps15 is also present at the trans-Golgi network (TGN). Therefore, we predicted that Eps15 might associate with clathrin adaptor proteins at the TGN and thereby mediate the formation of Golgi-derived vesicles. Indeed, we have found that Eps15 and the TGN clathrin adaptor AP-1 coimmunoprecipitate from rat liver Golgi fractions. Furthermore, we have identified a 14-amino acid motif near the AP-2-binding domain of Eps15 that is required for binding to AP-1, but not AP-2. Disruption of the Eps15-AP-1 interaction via siRNA knockdown of AP-1 or expression of mutant Eps15 protein, which lacks a 14-amino acid motif representing the AP-1 binding site of Eps15, significantly reduced the exit of secretory proteins from the TGN. Together, these findings indicate that Eps15 plays an important role in clathrin-coated vesicle formation not only at the plasma membrane but also at the TGN during the secretory process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.