ArticleBioorganic & medicinal chemistry letters2008
Quantitating the concentration of Py-Im polyamide-fluorescein conjugates in live cells.
Article in Bioorganic & medicinal chemistry letters, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing.Nucleic acids research · 2016Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Structure-based DNA-targeting strategies with small molecule ligands for drug discovery.Medicinal research reviews · 2013Review
- Synthesis of cyclic Py-Im polyamide libraries.The Journal of organic chemistry · 2013Article
- Characterization and solubilization of pyrrole-imidazole polyamide aggregates.Journal of medicinal chemistry · 2012Article
- Selective esterase-ester pair for targeting small molecules with cellular specificity.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Enhancing the cellular uptake of Py-Im polyamides through next-generation aryl turns.Nucleic acids research · 2012Article
- Allosteric analysis of glucocorticoid receptor-DNA interface induced by cyclic Py-Im polyamide: a molecular dynamics simulation study.PloS one · 2012Article
- Complexity in the binding of minor groove agents: netropsin has two thermodynamically different DNA binding modes at a single site.Nucleic acids research · 2011Article
- HPV episome levels are potently decreased by pyrrole-imidazole polyamides.Antiviral research · 2011Article
- Structural basis for cyclic Py-Im polyamide allosteric inhibition of nuclear receptor binding.Journal of the American Chemical Society · 2010Article
- Oligomerization route to Py-Im polyamide macrocycles.Organic letters · 2009Article
- Investigation of the relative cellular permeability of DNA-binding pyrrole-imidazole polyamides.Journal of medicinal chemistry · 2009Article
- Allosteric modulation of DNA by small molecules.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Solution-phase synthesis of pyrrole-imidazole polyamides.Journal of the American Chemical Society · 2009Article
- Cyclic pyrrole-imidazole polyamides targeted to the androgen response element.Journal of the American Chemical Society · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Quantitative fluorescence-based methods have been developed to determine the nuclear concentration of polyamide-fluorescein conjugates in cell culture. Confocal laser scanning microscopy and flow cytometry techniques are utilized to plot calibration curves, from which the nuclear concentration can be interpolated. Upon treatment with polyamide, the concentration in the nucleus of live HeLa cells is calculated to be between 0.1 and 0.5microM, which is significantly lower than the 2microM dosage concentration. In contrast, the observed nuclear concentration in U251 cells is closer to the dosage concentration, indicating a cell line-specific increase in uptake for this class of compounds. Although confocal microscopy and flow cytometry generate disparate values, taken together these experiments suggest that the polyamide concentration inside the cell nucleus is lower than it is outside the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.