ArticleJournal of controlled release : official journal of the Controlled Release Society2008
Evaluation of the effect of vector architecture on DNA condensation and gene transfer efficiency.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- Re-examination of Peptide-Sequence-Dependent Gene Expression of Cysteine-Installed Pegylated Oligolysine/DNA Complexes.ACS omega · 2022Article
- A Global Review on Short Peptides: Frontiers and Perspectives.Molecules (Basel, Switzerland) · 2021Review
- The Multifaceted Histidine-Based Carriers for Nucleic Acid Delivery: Advances and Challenges.Pharmaceutics · 2020Review
- Development of a Recombinant Multifunctional Biomacromolecule for Targeted Gene Transfer to Prostate Cancer Cells.Biomacromolecules · 2017Article
- Self-Assembly of a Multifunctional Lipid With Core-Shell Dendrimer DNA Nanoparticles Enhanced Efficient Gene Delivery at Low Charge Ratios into RPE Cells.Macromolecular bioscience · 2015Article
- Influence of histidine incorporation on buffer capacity and gene transfection efficiency of HPMA-co-oligolysine brush polymers.Biomacromolecules · 2013Article
- Hyaluronic acid based self-assembling nanosystems for CD44 target mediated siRNA delivery to solid tumors.Biomaterials · 2013Article
- Modified polyethyleneimine with histidine-lysine short peptides as gene carrier.Cancer gene therapy · 2011Article
- Development of recombinant cationic polymers for gene therapy research.Advanced drug delivery reviews · 2010Review
- Biosynthesis and characterization of a novel genetically engineered polymer for targeted gene transfer to cancer cells.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- A designer biomimetic vector with a chimeric architecture for targeted gene transfer.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers.British journal of pharmacology · 2009Review
- Perspectives in vector development for systemic cancer gene therapy.Gene therapy & molecular biology · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The objective of this study was to evaluate the effect of vector architecture on DNA condensation, particle stability, and gene transfer efficiency. Two recombinant non-viral vectors with the same amino acid compositions but different architectures, composed of lysine-histidine (KH) repeating units fused to fibroblast growth factor, were genetically engineered. In one vector lysine residues were dispersed (KHKHKHKHKK)(6)-FGF2, whereas in the other they were in clusters (KKKHHHHKKK)(6)-FGF2. Organization of lysine residues in this manner was inspired by the sequence of DNA condensing motifs that exist in nature (e.g., histones) where lysine residues are organized in clusters. These two constructs were compared in terms of DNA condensation and gene transfer efficiency. It was observed that the construct with KH units in clusters was able to condense pDNA into more stable particles with sizes <150 nm making them suitable for cellular uptake via receptor mediated endocytosis. This in turn resulted in five times higher transfection efficiency for the cKH-FGF2. This study demonstrates that in targeted non-viral gene transfer, the vector architecture plays as significant a role as its amino acid sequence. Thus, in the design of the non-viral vectors (synthetic or recombinant) this factor should be considered of paramount importance.
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Registered trials
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