ArticlePloS one2008
Bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Elevated MyoD1 levels expand genome-wide binding and the repertoire of regulated genes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Comparison of Tumour-Specific Phenotypes in Human Primary and Expandable Pancreatic Cancer Cell Lines.International journal of molecular sciences · 2023Article
- Synthetic rewiring and boosting type I interferon responses for visualization and counteracting viral infections.Nucleic acids research · 2020Article
- Antiviral activity of interleukin-11 as a response to porcine epidemic diarrhea virus infection.Veterinary research · 2019Article
- Model-based analysis of influenza A virus replication in genetically engineered cell lines elucidates the impact of host cell factors on key kinetic parameters of virus growth.PLoS computational biology · 2019Article
- Expansion of functional personalized cells with specific transgene combinations.Nature communications · 2018Article
- Cell Polarization and Epigenetic Status Shape the Heterogeneous Response to Type III Interferons in Intestinal Epithelial Cells.Frontiers in immunology · 2017Article
- Article
- Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut.Scientific reports · 2016Article
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- Stochastic simulation in systems biology.Computational and structural biotechnology journal · 2014Review
- Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions.PloS one · 2013Article
- Mapping the environmental fitness landscape of a synthetic gene circuit.PLoS computational biology · 2012Article
- Review
- Synthetic biology: mapping the scientific landscape.PloS one · 2012Article
- Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells.Nucleic acids research · 2011Article
- The type of responder T-cell has a significant impact in a human in vitro suppression assay.PloS one · 2010Article
- Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels.BMC cell biology · 2010Article
- Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.PloS one · 2010Article
- Coherent activation of a synthetic mammalian gene network.Systems and synthetic biology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to establish cells and organisms with predictable properties, synthetic biology makes use of controllable, synthetic genetic devices. These devices are used to replace or to interfere with natural pathways. Alternatively, they may be interlinked with endogenous pathways to create artificial networks of higher complexity. While these approaches have been already successful in prokaryotes and lower eukaryotes, the implementation of such synthetic cassettes in mammalian systems and even animals is still a major obstacle. This is mainly due to the lack of methods that reliably and efficiently transduce synthetic modules without compromising their regulation properties. To pave the way for implementation of synthetic regulation modules in mammalian systems we utilized lentiviral transduction of synthetic modules. A synthetic positive feedback loop, based on the Tetracycline regulation system was implemented in a lentiviral vector system and stably integrated in mammalian cells. This gene regulation circuit yields a bimodal expression response. Based on experimental data a mathematical model based on stochasticity was developed which matched and described the experimental findings. Modelling predicted a hysteretic expression response which was verified experimentally. Thereby supporting the idea that the system is driven by stochasticity. The results presented here highlight that the combination of three independent tools/methodologies facilitate the reliable installation of synthetic gene circuits with predictable expression characteristics in mammalian cells and organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.