ArticleJournal of immunology (Baltimore, Md. : 1950)2008
Platelet-activating factor-mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3-kinase in human neutrophils.
Article in Journal of immunology (Baltimore, Md. : 1950), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Phosphoinositol 3-kinase-driven NET formation involves different isoforms and signaling partners depending on the stimulus.Frontiers in immunology · 2023Article
- Glutamyl-prolyl-tRNA synthetase 1 coordinates early endosomal anti-inflammatory AKT signaling.Nature communications · 2022Article
- Regulation of platelet-activating factor-induced interleukin-8 expression by protein tyrosine phosphatase 1B.Cell communication and signaling : CCS · 2019Article
- Disruption of transient receptor potential melastatin 2 decreases elastase release and bacterial clearance in neutrophils.Innate immunity · 2018Article
- Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation.Transfusion · 2017Article
- Endocytosis is required for exocytosis and priming of respiratory burst activity in human neutrophils.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2017Article
- LysoPCs induce Hck- and PKCδ-mediated activation of PKCγ causing p47phox phosphorylation and membrane translocation in neutrophils.Journal of leukocyte biology · 2017Article
- Bacterial exploitation of phosphorylcholine mimicry suppresses inflammation to promote airway infection.The Journal of clinical investigation · 2015Article
- Clathrin complexes with the inhibitor kappa B kinase signalosome: imaging the interactome.Physiological reports · 2014Article
- Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases.Alzheimer's research & therapy · 2014Article
- Nitroarachidonic acid prevents NADPH oxidase assembly and superoxide radical production in activated macrophages.Free radical biology & medicine · 2013Article
- The subcellular localization of the receptor for platelet-activating factor in neutrophils affects signaling and activation characteristics.Clinical & developmental immunology · 2013Article
- β-Arrestins in the immune system.Progress in molecular biology and translational science · 2013Review
- Regulation of alveolar macrophage p40phox: hierarchy of activating kinases and their inhibition by PGE2.Journal of leukocyte biology · 2012Article
- CD13 regulates dendritic cell cross-presentation and T cell responses by inhibiting receptor-mediated antigen uptake.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent endosomal reactive oxygen species.The Journal of biological chemistry · 2012Article
- Early endosomal antigen 1 (EEA1) is an obligate scaffold for angiotensin II-induced, PKC-alpha-dependent Akt activation in endosomes.The Journal of biological chemistry · 2011Article
- Lysophosphatidylcholines activate G2A inducing G(αi)₋₁-/G(αq/)₁₁- Ca²(+) flux, G(βγ)-Hck activation and clathrin/β-arrestin-1/GRK6 recruitment in PMNs.The Biochemical journal · 2010Article
- GnRH signaling, the gonadotrope and endocrine control of fertility.Frontiers in neuroendocrinology · 2010Review
- Tumor necrosis factor-alpha causes release of cytosolic interleukin-18 from human neutrophils.American journal of physiology. Cell physiology · 2010Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Neutrophils (polymorphonuclear leukocytes, PMNs) are vital to innate immunity and receive proinflammatory signals that activate G protein-coupled receptors (GPCRs). Because GPCRs transduce signals through clathrin-mediated endocytosis (CME), we hypothesized that platelet-activating factor (PAF), an effective chemoattractant that primes the PMN oxidase, would signal through CME, specifically via dynamin-2 activation and endosomal formation resulting in membrane translocation of cytosolic phagocyte oxidase (phox) proteins. PMNs were incubated with buffer or 2 muM PAF for 1-3 min, and in some cases activated with PMA, and O(2)(-) was measured, whole-cell lysates and subcellular fractions were prepared, or the PMNs were fixed onto slides for digital or electron microscopy. PAF caused activation of dynamin-2, resulting in endosomal formation that required PI3K and contained early endosomal Ag-1 (EEA-1) and Rab5a. The apoptosis signal-regulating kinase-1/MAPK kinase-3/p38 MAPK signalosome assembled on Rab5a and phosphorylated EEA-1 and Rab GDP dissociation inhibitor, with the latter causing Rab5a activation. Electron microscopy demonstrated that PAF caused two distinct sites for activation of p38 MAPK. EEA-1 provided a scaffold for recruitment of the p40(phox)-p67(phox) complex and PI3K-dependent Akt1 phosphorylation of these two phox proteins. PAF induced membrane translocation of p40(phox)-p67(phox) localizing to gp91(phox), which was PI3K-, but not p47(phox)-, dependent. In conclusion, PAF transduces signals through CME, and such GPCR signaling may allow for pharmacological manipulation of these cells to decrease PMN-mediated acute organ injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.