ArticleProceedings of the National Academy of Sciences of the United States of America2008
Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
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- Two Decades of Arrayed Imaging Reflectometry for Sensitive, High-Throughput Biosensing.Biosensors · 2023Review
- Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection.Sensors (Basel, Switzerland) · 2023Review
- Two-Color iSCAT Imaging of Ag Nanoparticles Resolves Size and Ambient Refractive Index Changes.Nano letters · 2023Article
- Highly-Sensitive, Label-Free Detection of Microorganisms and Viruses via Interferometric Reflectance Imaging Sensor.Micromachines · 2023Review
- Sensitive surface plasmon resonance label-free biosensor on a fiber end-facet.Light, science & applications · 2022Article
- Label-free optical biosensors in the pandemic era.Nanophotonics (Berlin, Germany) · 2022Review
- Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions.Sensors (Basel, Switzerland) · 2022Article
- Recent advances in optical label-free characterization of extracellular vesicles.Nanophotonics (Berlin, Germany) · 2022Review
- Silver nanotriangle array based LSPR sensor for rapid coronavirus detection.Sensors and actuators. B, Chemical · 2022Article
- Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes.Nature communications · 2022Article
- Multiplexed, High-Sensitivity Measurements of Antibody Affinity Using Interferometric Reflectance Imaging Sensor.Biosensors · 2021Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Rapid and Easy-to-Use Method for Accurate Characterization of Target Binding and Kinetics of Magnetic Particle Bioconjugates for Biosensing.Sensors (Basel, Switzerland) · 2021Article
- A Reliable, Label Free Quality Control Method for the Production of DNA Microarrays with Clinical Applications.Polymers · 2021Article
- Plasmonic scattering imaging of single proteins and binding kinetics.Nature methods · 2020Article
- Engineering photonics solutions for COVID-19.APL photonics · 2020Review
- A Numerical Investigation of Enhancing Microfluidic Heterogeneous Immunoassay on Bipolar Electrodes Driven by Induced-Charge Electroosmosis in Rotating Electric Fields.Micromachines · 2020Article
Corrections and comments
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Authors and funding
8 authors.
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Abstract
Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm(2) SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.
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