ArticleJournal of cell science2008
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 67 citations in OpenAlex.
- Cryptic sex in Leishmania depends on SPO11 paralogs.PLoS pathogens · 2026Article
- Cryptic Sex inbioRxiv : the preprint server for biology · 2026Article
- Bromodomain protein IBD1 bridges histone acetylation and H2A.Z deposition to fine-tune transcription.Nucleic acids research · 2026Article
- A specialized TFIIB is required for transcription of transposon-targeting noncoding RNAs.Nucleic acids research · 2025Article
- Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning inMolecular biology of the cell · 2025Article
- The Hop2-Mnd1 Complex and Its Regulation of Homologous Recombination.Biomolecules · 2023Review
- A practical reference for studying meiosis in the model ciliateMarine life science & technology · 2022Review
- Zfp1, a Cys2His2 zinc finger protein is required for meiosis initiation inCell cycle (Georgetown, Tex.) · 2022Article
- Review
- Arrested crossover precursor structures form stable homologous bonds in a Tetrahymena meiotic mutant.PloS one · 2022Article
- The histone chaperone Nrp1 is required for chromatin stability and nuclear division in Tetrahymena thermophila.Epigenetics & chromatin · 2021Article
- Tetrahymena meiosis: Simple yet ingenious.PLoS genetics · 2021Review
- Article
- Phospho-Regulation of Meiotic Prophase.Frontiers in cell and developmental biology · 2021Review
- Spatial constraints on chromosomes are instrumental to meiotic pairing.Journal of cell science · 2020Article
- Rearrangement of macronucleus chromosomes correspond to TAD-like structures of micronucleus chromosomes inGenome research · 2020Article
- A distinct class of eukaryotic MT-A70 methyltransferases maintain symmetric DNA N6-adenine methylation at the ApT dinucleotides as an epigenetic mark associated with transcription.Nucleic acids research · 2019Article
- An MCM family protein promotes interhomolog recombination by preventing precocious intersister repair of meiotic DSBs.PLoS genetics · 2019Article
- A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation.Nucleic acids research · 2018Article
- A streamlined cohesin apparatus is sufficient for mitosis and meiosis in the protist Tetrahymena.Chromosoma · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
During meiosis, the micronuclei of the ciliated protist Tetrahymena thermophila elongate dramatically. Within these elongated nuclei, chromosomes are arranged in a bouquet-like fashion and homologous pairing and recombination takes place. We studied meiotic chromosome behavior in Tetrahymena in the absence of two genes, SPO11 and a homolog of HOP2 (HOP2A), which have conserved roles in the formation of meiotic DNA double-strand breaks (DSBs) and their repair, respectively. Single-knockout mutants for each gene display only a moderate reduction in chromosome pairing, but show a complete failure to form chiasmata and exhibit chromosome missegregation. The lack of SPO11 prevents the elongation of meiotic nuclei, but it is restored by the artificial induction of DSBs. In the hop2ADelta mutant, the transient appearance of gamma-H2A.X and Rad51p signals indicates the formation and efficient repair of DSBs; but this repair does not occur by interhomolog crossing over. In the absence of HOP2A, the nuclei are elongated, meaning that DSBs but not their conversion to crossovers are required for the development of this meiosis-specific morphology. In addition, by in silico homology searches, we compiled a list of likely Tetrahymena meiotic proteins as the basis for further studies of the unusual synaptonemal complex-less meiosis in this phylogenetically remote model organism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.