ArticleJournal of the Royal Society, Interface2008
A cellular isolation system for real-time single-cell oxygen consumption monitoring.
Article in Journal of the Royal Society, Interface, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Efficient single-cell oxygen consumption rate characterization based on frequency domain fluorescence lifetime imaging microscopy measurement and microfluidic platform.Biomicrofluidics · 2023Article
- A platform for high-throughput bioenergy production phenotype characterization in single cells.Scientific reports · 2017Article
- Microchambers with Solid-State Phosphorescent Sensor for Measuring Single Mitochondrial Respiration.Sensors (Basel, Switzerland) · 2016Article
- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Method for physiologic phenotype characterization at the single-cell level in non-interacting and interacting cells.Journal of biomedical optics · 2012Article
- Light-addressable measurements of cellular oxygen consumption rates in microwell arrays based on phase-based phosphorescence lifetime detection.Biomicrofluidics · 2012Article
- Cell-directed-assembly: directing the formation of nano/bio interfaces and architectures with living cells.Biochimica et biophysica acta · 2011Review
- Direct measurement of oxygen consumption rates from attached and unattached cells in a reversibly sealed, diffusionally isolated sample chamber.Advances in bioscience and biotechnology (Print) · 2010Article
- The role of physiological heterogeneity in microbial population behavior.Nature chemical biology · 2010Review
- Article
- New frontiers in single-cell analysis.Journal of the Royal Society, Interface · 2008Article
- A New Approach for Measuring Single-Cell Oxygen Consumption Rates.IEEE transactions on automation science and engineering : a publication of the IEEE Robotics and Automation Society · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
The development of a cellular isolation system (CIS) that enables the monitoring of single-cell oxygen consumption rates in real time is presented. The CIS was developed through a multidisciplinary effort within the Microscale Life Sciences Center (MLSC) at the University of Washington. The system comprises arrays of microwells containing Pt-porphyrin-embedded polystyrene microspheres as the reporter chemistry, a lid actuator system and a gated intensified imaging camera, all mounted on a temperature-stabilized confocal microscope platform. Oxygen consumption determination experiments were performed on RAW264.7 mouse macrophage cells as proof of principle. Repeatable and consistent measurements indicate that the oxygen measurements did not adversely affect the physiological state of the cells measured. The observation of physiological rates in real time allows studies of cell-to-cell heterogeneity in oxygen consumption rate to be performed. Such studies have implications in understanding the role of mitochondrial function in the progression of inflammatory-based diseases, and in diagnosing and treating such diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.