ArticleCell stem cell2008
High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
Article in Cell stem cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
84 citing papers in PubMed, 219 citations in OpenAlex.
- High-throughput screening for cell binding and repulsion peptides on multifunctionalized surfaces.Communications biology · 2024Article
- A targetable pathway to eliminate TRA-1-60+/TRA-1-81+ chemoresistant cancer cells.Journal of molecular cell biology · 2023Article
- Using high throughput screens to predict miscarriages with placental stem cells and long-term stress effects with embryonic stem cells.Birth defects research · 2022Review
- Cell-Based Double-Screening Method to Identify a Reliable Candidate for Osteogenesis-Targeting Compounds.Biomedicines · 2022Article
- Phenotypic technologies in stem cell biology.Cell chemical biology · 2021Review
- Small Molecule-Induced Pancreatic β-Like Cell Development: Mechanistic Approaches and Available Strategies.International journal of molecular sciences · 2020Review
- Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture.Stem cell reports · 2020Article
- Down-regulation of ATF1 leads to early neuroectoderm differentiation of human embryonic stem cells by increasing the expression level of SOX2.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- DRUGPATH - a novel bioinformatic approach identifies DNA-damage pathway as a regulator of size maintenance in human ESCs and iPSCs.Scientific reports · 2019Article
- High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture.Biotechnology and bioengineering · 2019Article
- Shen-Jing as a Chinese Medicine Concept Might Be a Counterpart of Stem Cells in Regenerative Medicine.Chinese journal of integrative medicine · 2019Article
- Screening-Based Chemical Approaches to Unravel Stem Cell Biology.Stem cell reports · 2018Review
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- Teratogen screening with human pluripotent stem cells.Integrative biology : quantitative biosciences from nano to macro · 2018Review
- In Vitro Microscale Models for Embryogenesis.Advanced biosystems · 2018Article
- Chronic lung disease, lung regeneration and future therapeutic strategies.Chronic diseases and translational medicine · 2018Article
- Elimination of undifferentiated human embryonic stem cells by cardiac glycosides.Scientific reports · 2017Article
- High content analysis in amyotrophic lateral sclerosis.Molecular and cellular neurosciences · 2017Review
- Article
- Cardiomyocytes from human pluripotent stem cells: From laboratory curiosity to industrial biomedical platform.Biochimica et biophysica acta · 2016Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
High-throughput screening (HTS) of chemical libraries has become a critical tool in basic biology and drug discovery. However, its implementation and the adaptation of high-content assays to human embryonic stem cells (hESCs) have been hampered by multiple technical challenges. Here we present a strategy to adapt hESCs to HTS conditions, resulting in an assay suitable for the discovery of small molecules that drive hESC self-renewal or differentiation. Use of this new assay has led to the identification of several marketed drugs and natural compounds promoting short-term hESC maintenance and compounds directing early lineage choice during differentiation. Global gene expression analysis upon drug treatment defines known and novel pathways correlated to hESC self-renewal and differentiation. Our results demonstrate feasibility of hESC-based HTS and enhance the repertoire of chemical compounds for manipulating hESC fate. The availability of high-content assays should accelerate progress in basic and translational hESC biology.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.