ArticlePlant cell reports2008
Transformation by T-DNA integration causes highly sterile phenotype independent of transgenes in Arabidopsis thaliana.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination.Plant cell reports · 2019Review
- Germline-transmitted genome editing in Arabidopsis thaliana Using TAL-effector-nucleases.PloS one · 2015Article
- BioVector, a flexible system for gene specific-expression in plants.BMC plant biology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens-mediated gene transformation caused highly sterile phenotype in T1 transgenic populations of Arabidopsis thaliana. The phenomenon occurred independent of the genes and construct types used for transformation. The occurring frequency is less than 10% and the phenotype is inheritable. Intensive examination elucidated that the sterility is due to indehiscence or delayed dehiscence of the anthers during the flowering stage, resulting from the reduced or disordered endothecial secondary wall thickening of the anthers in the sterile flowers. Exogenous jasmonic acid application cannot rescue the sterile phenotype. Additionally, by using the Escherichia coli uidA gene encoding the beta-glucuronidase as a reporter gene, we indicated that the Cauliflower mosaic virus 35S promoter was not constitutively active as expected previously in the reproductive organs of Arabidopsis. These results contribute significantly to the plant community by suggesting that more careful examination and statistical analysis are needed while studying gain-of-function phenotypes of genes, especially for genes that might be involved in reproductive development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.