ArticleThe Journal of biological chemistry2008
The viral protein Egf1.0 is a dual activity inhibitor of prophenoloxidase-activating proteinases 1 and 3 from Manduca sexta.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Transforming hosts into nurseries and nutrients: strategic manipulation by endoparasitoid wasps.Journal of advanced research · 2026Review
- A chromosome-scale genome assembly and functional studies implicate the viral RNA polymerase in transcription of most Microplitis demolitor bracovirus late genes.Journal of virology · 2026Article
- Innate Immunity in Insects: The Lights and Shadows of Phenoloxidase System Activation.International journal of molecular sciences · 2025Review
- Two venom serpins from the parasitoid wasp Microplitis mediator inhibit the host prophenoloxidase activation and antimicrobial peptide synthesis.Insect biochemistry and molecular biology · 2023Article
- Dual roles and evolutionary implications of P26/poxin in antagonizing intracellular cGAS-STING and extracellular melanization immunity.Nature communications · 2022Article
- A teratocyte-specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase-activating system of its host Plutella xylostella.Insect molecular biology · 2022Article
- The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation.Frontiers in immunology · 2022Article
- MdBVe46 is an envelope protein that is required for virion formation by Microplitis demolitor bracovirus.The Journal of general virology · 2021Article
- Changes in composition and levels of hemolymph proteins during metamorphosis of Manduca sexta.Insect biochemistry and molecular biology · 2020Article
- Identification of a Conserved Prophenoloxidase Activation Pathway in Cotton BollwormFrontiers in immunology · 2020Article
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- Inhibition of Spodoptera frugiperda phenoloxidase activity by the products of the Xenorhabdus rhabduscin gene cluster.PloS one · 2019Article
- Inhibition of melanization by serpin-5 and serpin-9 promotes baculovirus infection in cotton bollworm Helicoverpa armigera.PLoS pathogens · 2017Article
- A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases.The Journal of biological chemistry · 2017Article
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- Article
- Analysis of Genetic Variation across the Encapsidated Genome of Microplitis demolitor Bracovirus in Parasitoid Wasps.PloS one · 2016Article
- Suppression of shrimp melanization during white spot syndrome virus infection.The Journal of biological chemistry · 2015Article
- Caspase-1-like regulation of the proPO-system and role of ppA and caspase-1-like cleaved peptides from proPO in innate immunity.PLoS pathogens · 2014Article
- Systematic analysis of a wasp parasitism arsenal.Molecular ecology · 2014Article
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5 authors.
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Abstract
Some pathogens are capable of suppressing the melanization response of host insects, but the virulence factors responsible are largely unknown. The insect pathogen Microplitis demolitor bracovirus encodes the Egf family of small serine proteinase inhibitors. One family member, Egf1.0, was recently shown to suppress melanization of hemolymph in Manduca sexta in part by inhibiting the enzymatic activity of prophenoloxidase activating proteinase 3 (PAP3). However, other experiments suggested this viral protein suppresses melanization by more than one mechanism. Here we report that Egf1.0 inhibited the amidolytic activity of PAP1 and dose-dependently blocked processing of pro-PAP1 and pro-PAP3. Consistent with its PAP inhibitory activity, Egf1.0 also prevented processing of pro-phenoloxidase, serine proteinase homolog (SPH) 1, and SPH2. Isolation of Egf1.0-protein complexes from plasma indicated that Egf1.0 binds PAPs through its C-terminal repeat domain. Egf1.0 also potentially interacts with SPH2 and two other proteins, ferritin and gloverin, not previously associated with the phenoloxidase cascade. Overall, our results indicate that Egf1.0 is a dual activity PAP inhibitor that strongly suppresses the insect melanization response.
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