ArticleClinical orthopaedics and related research2008
Metastatic osteosarcoma gene expression differs in vitro and in vivo.
Article in Clinical orthopaedics and related research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Matrix type influences embedded patient-derived osteosarcoma organoid invasion and response to treatment.Frontiers in pharmacology · 2026Article
- Vitamin D inhibits osteosarcoma by reprogramming nonsense-mediated RNA decay and SNAI2-mediated epithelial-to-mesenchymal transition.Frontiers in oncology · 2023Article
- 12 Survival-related differentially expressed genes based on the TARGET-osteosarcoma database.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Drug Tolerant Cells: An Emerging Target With Unique Transcriptomic Features.Cancer informatics · 2019Article
- Effect of low- and high-linear energy transfer radiation on in vitro and orthotopic in vivo models of osteosarcoma by activation of caspase-3 and -9.International journal of oncology · 2017Article
- Osteosarcoma tissues and cell lines from patients with differing serum alkaline phosphatase concentrations display minimal differences in gene expression patterns.Veterinary and comparative oncology · 2016Article
- Natural compounds for pediatric cancer treatment.Naunyn-Schmiedeberg's archives of pharmacology · 2016Review
- Genetically engineered mouse models and human osteosarcoma.Clinical sarcoma research · 2012Article
- Targeting radioresistant osteosarcoma cells with parthenolide.Journal of cellular biochemistry · 2012Article
- [Diagnostic imaging and visualization of radiation treatment effects in soft tissue sarcomas].Der Radiologe · 2012Article
- Characterization of human multicentric osteosarcoma using newly established cells derived from multicentric osteosarcoma.Journal of cancer research and clinical oncology · 2011Article
- Driven to death: Inhibition of farnesylation increases Ras activity and promotes growth arrest and cell death [corrected].Molecular cancer therapeutics · 2010Article
- An orthotopic, postsurgical model of luciferase transfected murine osteosarcoma with spontaneous metastasis.Clinical & experimental metastasis · 2010Article
- [Imaging of soft tissue sarcomas: standard approaches and new strategies].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2009Review
- A clinically relevant mouse model of canine osteosarcoma with spontaneous metastasis.In vivo (Athens, Greece)Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An understanding of differential gene expression in highly metastatic osteosarcoma could provide gene targets for treatment of metastases. We compared gene expression profiles of high- (LM7) and low- (LM2) metastatic SaOS2-derived cell lines in an in vitro tissue culture model and examined several differentially regulated genes in vivo in a murine orthotopic xenograft model. We hypothesized an orthotopic inoculation of LM2 and LM7 cells would establish a primary lesion and the gene expression profile of cells grafted in this fashion would resemble the gene expression profile observed in an in vitro model. Thirty-five days after inoculation, animals were euthanized and both tibiae were harvested and rapidly frozen in liquid nitrogen. Human-specific GAPDH mRNA was present in two of four tibias inoculated with LM2 cells and three of four tibias inoculated with LM7 cells. Tibiae displaying the presence of human cells were assayed by semiquantitative reverse transcriptase polymerase chain reaction. We observed poor correspondence of in vitro to in vivo gene expression for either cell line. Accordingly, in vitro osteosarcoma gene expression data must be interpreted with caution until confirmed in vivo. Our orthotopic injection model allowed in vivo study of differential gene expression between these two cell lines but did not show radiographic evidence of an established primary lesion.
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