ArticleMolecular genetics and genomics : MGG2008
Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii.
Article in Molecular genetics and genomics : MGG, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Current Nuclear Engineering Strategies in the Green MicroalgaLife (Basel, Switzerland) · 2023Review
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- A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo.Nature communications · 2023Article
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- Micronutrient optimization for tissue engineered articular cartilage production of type II collagen.Frontiers in bioengineering and biotechnology · 2023Article
- Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression inFrontiers in plant science · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Validated Nuclear-Based Transgene Expression Regulated by the Fea1 Iron-Responsive Promoter in the Green Alga Chlamydomonas reinhardtii.Molecular biotechnology · 2019Article
- Article
- Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.The Plant journal : for cell and molecular biology · 2017Article
- High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii.Plant biotechnology journal · 2017Article
- Gene Regulatory Networks for the Haploid-to-Diploid Transition ofPlant physiology · 2017Article
- UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems.Plant molecular biology · 2016Article
- Identification and Characterization of a cis-Regulatory Element for Zygotic Gene Expression in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2016Article
- A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division.eLife · 2016Article
- Establishing Chlamydomonas reinhardtii as an industrial biotechnology host.The Plant journal : for cell and molecular biology · 2015Review
- TETX: a novel nuclear selection marker for Chlamydomonas reinhardtii transformation.Plant methods · 2015Article
- Transplantation of Human Neural Progenitor Cells Expressing IGF-1 Enhances Retinal Ganglion Cell Survival.PloS one · 2015Article
- A cell-based high-throughput approach to identify inhibitors of influenza A virus.Acta pharmaceutica Sinica. B · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the model organism Chlamydomonas reinhardtii, a codon-adapted gene variant of the extracellular luciferase of Gaussia princeps was generated as a sensitive molecular tool to study gene expression from the nuclear genome. In the past, monitoring promoter activity in Chlamydomonas employing the commonly used luciferase encoded by Renilla reniformis was hampered due to the detection limit of the reporter assay, especially if analyzing weak promoters. In this work, the expression of Gaussia-luciferase from such promoters resulted in an average luminescent activity at least 500 times higher than that detected for the Renilla enzyme. The wildtype signal peptide of Gaussia princeps efficiently mediated the export of the luciferase into the culture medium of Chlamydomonas strain cw15arg ( - ), and the characterization of the secreted protein showed an unexpected temperature instability, probably arising from post-translational modifications made by the algae. To further test the utility of Gaussia-luciferase, promoter sequences originating from different viral genomes were analyzed for their ability to drive transgene expression in Chlamydomonas. Solely, the 35S-promoter of the Cauliflower mosaic virus (CaMV) displayed a significant transcriptional activity and this happened only when the shunting region of the 5'-untranslated region of the 35S-sequence was omitted from the luciferase expression cassette. Gaussia-luciferase proved to be a superior quantifiable reporter gene for the analysis of constitutive promoter sequences in Chlamydomonas reinhardtii.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.