ArticleEuropean journal of applied physiology2008
Determination of myoglobin concentration in blood-perfused tissue.
Article in European journal of applied physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- A versatile delivery vehicle for cellular oxygen and fuels or metabolic sensor? A review and perspective on the functions of myoglobin.Physiological reviews · 2024Review
- Biosensing Platforms for Cardiac Biomarker Detection.ACS omega · 2024Review
- Spectroscopic identification of the catalytic intermediates of cytochrome c oxidase in respiring heart mitochondria.Biochimica et biophysica acta. Bioenergetics · 2023Article
- Metabolic physiology and skeletal muscle phenotypes in male and female myoglobin knockout mice.American journal of physiology. Endocrinology and metabolism · 2021Article
- Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion.Analytical biochemistry · 2019Article
- Differential Interaction of Myoglobin with Select Fatty Acids of Carbon Chain Lengths C8 to C16.Lipids · 2017Article
- Nitric oxide release by deoxymyoglobin nitrite reduction during cardiac ischemia: A mathematical model.Microvascular research · 2017Article
- Effect of fatty acid interaction on myoglobin oxygen affinity and triglyceride metabolism.Journal of physiology and biochemistry · 2016Article
- Interaction of myoglobin with oleic acid.Chemistry and physics of lipids · 2015Article
- Endurance training facilitates myoglobin desaturation during muscle contraction in rat skeletal muscle.Scientific reports · 2015Article
- Palmitate interaction with physiological states of myoglobin.Biochimica et biophysica acta · 2014Article
- The continuing evolution of the Langendorff and ejecting murine heart: new advances in cardiac phenotyping.American journal of physiology. Heart and circulatory physiology · 2012Review
- Myoglobin's old and new clothes: from molecular structure to function in living cells.The Journal of experimental biology · 2010Review
- Endogenous myoglobin in human breast cancer is a hallmark of luminal cancer phenotype.British journal of cancer · 2010Article
- Interaction of fatty acid with myoglobin.FEBS letters · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The standard method for determining the myoglobin (Mb) concentration in blood-perfused tissue often relies on a simple but clever differencing algorithm of the optical spectra, as proposed by Reynafarje. However, the underlying assumptions of the differencing algorithm do not always lead to an accurate assessment of Mb concentration in blood-perfused tissue. Consequently, the erroneous data becloud the understanding of Mb function and oxygen transport in the cell. The present study has examined the Mb concentration in buffer and blood-perfused mouse heart. In buffer-perfused heart containing no hemoglobin (Hb), the optical differencing method yields a tissue Mb concentration of 0.26 mM. In blood-perfused tissue, the method leads to an overestimation of Mb. However, using the distinct (1)H NMR signals of MbCO and HbCO yields a Mb concentration of 0.26 mM in both buffer- and blood-perfused myocardium. Given the NMR and optical data, a computer simulation analysis has identified some error sources in the optical differencing algorithm and has suggested a simple modification that can improve the Mb determination. Even though the present study has determined a higher Mb concentration than previously reported, it does not alter significantly the equipoise PO(2), the PO(2) where Mb and O(2) contribute equally to the O(2) flux. It also suggests that any Mb increase with exercise training does not necessarily enhance the intracellular O(2) delivery.
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