ArticleNucleic acids research1991
Eukaryotic topoisomerase I-DNA interaction is stabilized by helix curvature.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 69 citations in OpenAlex.
- Tricking enzymes in living cells: a mechanism-based strategy for design of DNA topoisomerase biosensors.Journal of nanobiotechnology · 2021Article
- Mapping topoisomerase sites in mitochondrial DNA with a poisonous mitochondrial topoisomerase I (Top1mt).The Journal of biological chemistry · 2014Article
- The stability of a model substrate for topoisomerase 1-mediated DNA religation depends on the presence of mismatched base pairs.Journal of nucleic acids · 2011Article
- A functional type I topoisomerase from Pseudomonas aeruginosa.BMC molecular biology · 2009Article
- Topoisomerase I-mediated DNA cleavage induced by the minor groove-directed binding of bibenzimidazoles to a distal site.Journal of molecular biology · 2007Article
- Position- and orientation-specific enhancement of topoisomerase I cleavage complexes by triplex DNA structures.Nucleic acids research · 2004Article
- p53 stimulates human topoisomerase I activity by modulating its DNA binding.Nucleic acids research · 2003Article
- Spatial organization of topoisomerase I-mediated DNA cleavage induced by camptothecin-oligonucleotide conjugates.Nucleic acids research · 2003Article
- The unusual structures of the hot-regions flanking large-scale deletions in human mitochondrial DNA.The Biochemical journal · 1996Article
- Preferential binding of human topoisomerase I to superhelical DNA.The EMBO journal · 1995Article
- Selective inhibition of topoisomerases from Pneumocystis carinii compared with that of topoisomerases from mammalian cells.Antimicrobial agents and chemotherapy · 1994Article
- Non-recombinant background in gene targeting: illegitimate recombination between a hpt gene and a defective 5' deleted nptII gene can restore a Kmr phenotype in tobacco.Plant molecular biology · 1994Article
- Topoisomerase I action on the heterochromatic DNA from the brine shrimp Artemia franciscana: studies in vivo and in vitro.The Biochemical journal · 1994Article
- DNA binding by the archaeal histone HMf results in positive supercoiling.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of DNA structure on topoisomerase I-DNA interaction has been investigated using a high affinity binding site and mutant derivatives thereof. Parallel determinations of complex formation and helix structure in the absence of superhelical stress suggest that the interaction is intensified by stable helix curvature. Previous work showed that a topoisomerase I binding site consists of two functionally distinct subdomains. A region located 5' to the topoisomerase I cleavage site is essential for binding. The region 3' to the cleavage site is covered by the enzyme, but not essential. We report here that the helix conformation of the latter region is an important modulator of complex formation. Thus, complex formation is markedly stimulated, when an intrinsically bent DNA segment is installed in this region. A unique pattern of phosphate ethylation interferences in the 3'-part of the binding site indicates that sensing of curvature involves backbone contacts. Since dynamic curvature in supercoiled DNA may substitute for stable curvature, our findings suggest that topoisomerase I is able to probe DNA topology by assessment of writhe, rather than twist.
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