ArticleApplied and environmental microbiology2008
Gamma irradiation can be used to inactivate Bacillus anthracis spores without compromising the sensitivity of diagnostic assays.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
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- Physiological Responses to Multiple Low-Doses ofPathogens (Basel, Switzerland) · 2020Article
- Physiological Responses to a Single Low-Dose ofPathogens (Basel, Switzerland) · 2020Article
- Review of Decontamination Techniques for the Inactivation of Bacillus anthracis and Other Spore-Forming Bacteria Associated with Building or Outdoor Materials.Environmental science & technology · 2019Review
- A Standard Method To Inactivate Bacillus anthracis Spores to Sterility via Gamma Irradiation.Applied and environmental microbiology · 2018Article
- Evaluation of Gamma-Radiation Inactivation of a Bioterrorism Agent,Indian journal of microbiology · 2018Article
- Whole-Genome Sequencing in Microbial Forensic Analysis of Gamma-Irradiated Microbial Materials.Applied and environmental microbiology · 2016Article
- Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial.Journal of clinical microbiology · 2015Article
- Fighting Ebola with novel spore decontamination technologies for the military.Frontiers in microbiology · 2015Article
- Bacillus thuringiensis as a surrogate for Bacillus anthracis in aerosol research.World journal of microbiology & biotechnology · 2014Review
- Benefits of a European project on diagnostics of highly pathogenic agents and assessment of potential "dual use" issues.Frontiers in public health · 2014Review
- Swab protocol for rapid laboratory diagnosis of cutaneous anthrax.Journal of clinical microbiology · 2012Article
- The role of nuclear technologies in the diagnosis and control of livestock diseases--a review.Tropical animal health and production · 2012Review
- Statement summarising the Conclusions and Recommendations from the Opinions on the Safety of Irradiation of Food adopted by the BIOHAZ and CEF Panels.EFSA journal. European Food Safety Authority · 2011Article
- Scientific Opinion on the efficacy and microbiological safety of irradiation of food.EFSA journal. European Food Safety Authority · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of Bacillus anthracis as a biological weapon in 2001 heightened awareness of the need for validated methods for the inactivation of B. anthracis spores. This study determined the gamma irradiation dose for inactivating virulent B. anthracis spores in suspension and its effects on real-time PCR and antigen detection assays. Strains representing eight genetic groups of B. anthracis were exposed to gamma radiation, and it was found that subjecting spores at a concentration of 10(7) CFU/ml to a dose of 2.5 x 10(6) rads resulted in a 6-log-unit reduction of spore viability. TaqMan real-time PCR analysis of untreated versus irradiated Ames strain (K1694) spores showed that treatment significantly enhanced the detection of B. anthracis chromosomal DNA targets but had no significant effect on the ability to detect targets on the pXO1 and pXO2 plasmids of B. anthracis. When analyzed by an enzyme-linked immunosorbent assay (ELISA), irradiation affected the detection of B. anthracis spores in a direct ELISA but had no effect on the limit of detection in a sandwich ELISA. The results of this study showed that gamma irradiation-inactivated spores can be tested by real-time PCR or sandwich ELISA without decreasing the sensitivity of either type of assay. Furthermore, the results suggest that clinical and public health laboratories which test specimens for B. anthracis could potentially incorporate gamma irradiation into sample processing protocols without compromising the sensitivity of the B. anthracis assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.