ArticleApplied and environmental microbiology2008
Short peptide induces an "uncultivable" microorganism to grow in vitro.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed.
- Article
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- Biodiversity and Biological Interactions of Actinobacteria Associated with Deep Sea and Intertidal Marine Invertebrates.Marine drugs · 2025Article
- Strategies Used for the Discovery of New Microbial Metabolites with Antibiotic Activity.Molecules (Basel, Switzerland) · 2025Review
- A Comprehensive Review on Bioactive Molecules and Advanced Microorganism Management Technologies.Current issues in molecular biology · 2024Review
- Impact ofEngineering microbiology · 2024Article
- The Marine Fish Gut Microbiome as a Source of Novel Bacteriocins.Microorganisms · 2024Review
- Modeling Dynamics of Human Gut Microbiota Derived from Gluten Metabolism: Obtention, Maintenance and Characterization of Complex Microbial Communities.International journal of molecular sciences · 2024Article
- Unlocking the potential of bacterial endophytes from medicinal plants for drug discovery.Microbial biotechnology · 2024Review
- Review
- Returning to Nature for the Next Generation of Antimicrobial Therapeutics.Antibiotics (Basel, Switzerland) · 2023Review
- Uncultured Microorganisms and Their Functions in the Fermentation Systems of Traditional Chinese Fermented Foods.Foods (Basel, Switzerland) · 2023Review
- Microbial Interaction is Among the Key Factors for Isolation of Previous Uncultured Microbes.Journal of microbiology (Seoul, Korea) · 2023Review
- Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons.Frontiers in microbiology · 2023Review
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- The identification of the new speciesFrontiers in microbiology · 2023Article
- Identification of a growth factor required for culturing specific fastidious oral bacteria.Journal of oral microbiology · 2023Article
- Marine Bioprospecting, Biocatalysis and Process Development.Microorganisms · 2022Review
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganisms comprise the bulk of biodiversity, but only a small fraction of this diversity grows on artificial media. This phenomenon was noticed almost a century ago, repeatedly confirmed, and termed the "great plate count anomaly." Advances in microbial cultivation improved microbial recovery but failed to explain why most microbial species do not grow in vitro. Here we show that at least some of such species can form domesticated variants capable of growth on artificial media. We also present evidence that small signaling molecules, such as short peptides, may be essential factors in initiating growth of nongrowing cells. We identified one 5-amino-acid peptide, LQPEV, that at 3.5 nM induces the otherwise "uncultivable" strain Psychrobacter sp. strain MSC33 to grow on standard media. This demonstrates that the restriction preventing microbial in vitro growth may be different from those offered to date to explain the "great plate count anomaly," such as deficiencies in nutrient composition and concentrations in standard media, medium toxicity, and inappropriate incubation time. Growth induction of MSC33 illustrates that some microorganisms do not grow in vitro because they are removed from their native communities and the signals produced therein. "Uncultivable" species represent the largest source of unexplored biodiversity, and provide remarkable opportunities for both basic and applied research. Access to cultures of some of these species should be possible through identification of the signaling compounds necessary for growth, their addition to standard medium formulations, and eventual domestication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.