ArticleBiophysical journal2008
Galphaq binds two effectors separately in cells: evidence for predetermined signaling pathways.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Neuroprotection against beta-amyloid toxicity by the novel estrogen receptor modulator STX requires convergent signaling pathways.Frontiers in molecular neuroscience · 2025Article
- Regulation of PI3K by PKC and MARCKS: Single-Molecule Analysis of a Reconstituted Signaling Pathway.Biophysical journal · 2016Article
- PLCβ1-SHP-2 complex, PLCβ1 tyrosine dephosphorylation and SHP-2 phosphatase activity: a new part of Angiotensin II signaling?Journal of biomedical science · 2011Article
- Differential response to morphine of the oligomeric state of μ-opioid in the presence of δ-opioid receptors.Biochemistry · 2011Article
- Identification of a novel binding partner of phospholipase cβ1: translin-associated factor X.PloS one · 2010Article
- The small G protein Rac1 activates phospholipase Cdelta1 through phospholipase Cbeta2.The Journal of biological chemistry · 2010Article
- G alpha(q) acts as an adaptor protein in protein kinase C zeta (PKCzeta)-mediated ERK5 activation by G protein-coupled receptors (GPCR).The Journal of biological chemistry · 2010Article
- The effect of membrane domains on the G protein-phospholipase Cbeta signaling pathway.Critical reviews in biochemistry and molecular biology · 2010Review
- Phospholipase Cbeta4 isozyme is expressed in human, rat, and murine heart left ventricles and in HL-1 cardiomyocytes.Molecular and cellular biochemistry · 2010Article
- Gq-coupled purinergic receptors inhibit insulin-like growth factor-I/phosphoinositide 3-kinase pathway-dependent keratinocyte migration.Molecular biology of the cell · 2010Article
- Evidence for a second, high affinity Gbetagamma binding site on Galphai1(GDP) subunits.The Journal of biological chemistry · 2009Article
- Coupling mode of receptors and G proteins.Naunyn-Schmiedeberg's archives of pharmacology · 2009Review
- A self-scaffolding model for G protein signaling.Journal of molecular biology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
G-proteins transduce signals along diverse pathways, but the factors involved in pathway selection are largely unknown. Here, we have studied the ability of Galpha(q) to select between two effectors-mammalian inositide-specific phospholipase Cbeta (PLCbeta) and phosphoinositide-3-kinase (PI3K)-in human embryonic kidney 293 cells. These studies were carried out by measuring interactions between eCFP- and eYFP-tagged proteins using Forster resonance energy transfer in the basal state and during stimulation. Instead of association of Galpha(q) with effectors through diffusion and exchange, we found separate and stable pools of Galpha(q)-PLCbeta and Galpha(q)-PI3K complexes existing throughout the stimulation cycle. These separate complexes existed despite the ability of Galpha(q) to simultaneously bind both effectors as determined by in vitro measurements using purified proteins. Preformed G-protein/effector complexes will limit the number of pathways that a given signal will take, which may simplify predictive models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.