ArticleNucleic acids research2008
Inversing the natural hydrogen bonding rule to selectively amplify GC-rich ADAR-edited RNAs.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Genomic characterization of avian metapneumovirus subtypes A and B in United States poultry by targeted amplicon sequencing.Frontiers in cellular and infection microbiology · 2026Article
- SARS-CoV-2-induced dysregulation in ADAR editing patterns persists post viral clearance in individuals with mild COVID-19.Frontiers in cellular and infection microbiology · 2026Article
- Bioinformatics for Inosine: Tools and Approaches to Trace This Elusive RNA Modification.Genes · 2024Review
- Review
- Quasispecies Nature of RNA Viruses: Lessons from the Past.Vaccines · 2023Review
- Porcine epidemic diarrhea virus strain FJzz1 infection induces type I/III IFNs production through RLRs and TLRs-mediated signaling.Frontiers in immunology · 2022Article
- ADAR Editing in Viruses: An Evolutionary Force to Reckon with.Genome biology and evolution · 2021Review
- Adenosine Deaminases Acting on RNA (ADARs) and Viral Infections.Annual review of virology · 2021Article
- Development of a Novel Class of Self-Assembling dsRNA Cancer Therapeutics: A Proof-of-Concept Investigation.Molecular therapy oncolytics · 2020Article
- The p150 Isoform of ADAR1 Blocks Sustained RLR signaling and Apoptosis during Influenza Virus Infection.PLoS pathogens · 2020Article
- RNA editing in the forefront of epitranscriptomics and human health.Journal of translational medicine · 2019Review
- Genotoxic stress increases cytoplasmic mitochondrial DNA editing by human APOBEC3 mutator enzymes at a single cell level.Scientific reports · 2019Article
- Mechanisms and Concepts in RNA Virus Population Dynamics and Evolution.Annual review of virology · 2018Review
- COLD-PCR Technologies in the Area of Personalized Medicine: Methodology and Applications.Molecular diagnosis & therapy · 2017Review
- Mechanisms of viral mutation.Cellular and molecular life sciences : CMLS · 2016Review
- Human norovirus hyper-mutation revealed by ultra-deep sequencing.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2016Article
- Extremely High Mutation Rate of HIV-1 In Vivo.PLoS biology · 2015Article
- Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.Clinical chemistry · 2015Article
- ADAR1 enhances HTLV-1 and HTLV-2 replication through inhibition of PKR activity.Retrovirology · 2014Article
- MicroRNA editing facilitates immune elimination of HCMV infected cells.PLoS pathogens · 2014Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA complementarity is expressed by way of three hydrogen bonds for a G:C base pair and two for A:T. As a result, careful control of the denaturation temperature of PCR allows selective amplification of AT-rich alleles. Yet for the same reason, the converse is not possible, selective amplification of GC-rich alleles. Inosine (I) hydrogen bonds to cytosine by two hydrogen bonds while diaminopurine (D) forms three hydrogen bonds with thymine. By substituting dATP by dDTP and dGTP by dITP in a PCR reaction, DNA is obtained in which the natural hydrogen bonding rule is inversed. When PCR is performed at limiting denaturation temperatures, it is possible to recover GC-rich viral genomes and inverted Alu elements embedded in cellular mRNAs resulting from editing by dsRNA dependent host cell adenosine deaminases. The editing of Alu elements in cellular mRNAs was strongly enhanced by type I interferon induction indicating a novel link mRNA metabolism and innate immunity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.