ArticleBiomaterials2008
Immunoproteomic identification of bovine pericardium xenoantigens.
Article in Biomaterials, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- The Ratio of Immunogenicity: A quantitative metric to identify highly immunogenic protein antigens to improve tissue-engineered biomaterials.Biomaterials · 2026Article
- Identification of Antibody-Cognate Antigens via a High-Throughput, Affinity Chromatography-Based, Shotgun Immunoproteomics Pipeline.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Reduction in Xenogeneic Epitopes on Porcine Endothelial Cells by Periodate Oxidation.Biomedicines · 2024Article
- Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification.Journal of proteomics · 2023Article
- Unraveling the Relevance of Tissue-Specific Decellularized Extracellular Matrix Hydrogels for Vocal Fold Regenerative Biomaterials: A Comprehensive Proteomic and In Vitro Study.Advanced nanobiomed research · 2023Article
- Functional acellular matrix for tissue repair.Materials today. Bio · 2023Review
- GTKO rabbit: A novel animal model for preclinical assessment of decellularized xenogeneic grafts via in situ implantation.Materials today. Bio · 2023Article
- The clinical effectiveness of staple line reinforcement with different matrix used in surgery.Frontiers in bioengineering and biotechnology · 2023Review
- Tissue engineered bovine saphenous vein extracellular matrix scaffolds produced via antigen removal achieve high in vivo patency rates.Acta biomaterialia · 2021Article
- Proteomic Analysis of Porcine-Derived Collagen Membrane and Matrix.Materials (Basel, Switzerland) · 2020Article
- Antigen removal process preserves function of small diameter venous valved conduits, whereas SDS-decellularization results in significant valvular insufficiency.Acta biomaterialia · 2020Article
- Small Diameter Xenogeneic Extracellular Matrix Scaffolds for Vascular Applications.Tissue engineering. Part B, Reviews · 2020Review
- Decellularization of Human Pericardium with Potential Application in Regenerative Medicine.Arquivos brasileiros de cardiologia · 2019Article
- In Depth Quantification of Extracellular Matrix Proteins from Human Pancreas.Journal of proteome research · 2019Article
- Immunoproteomic Identification of Noncarbohydrate Antigens Eliciting Graft-Specific Adaptive Immune Responses in Patients with Bovine Pericardial Bioprosthetic Heart Valves.Proteomics. Clinical applications · 2019Article
- Pericardial tissue for cardiovascular application: an in-vitro evaluation of established and advanced production processes.Journal of materials science. Materials in medicine · 2018Article
- Graft-specific immune tolerance is determined by residual antigenicity of xenogeneic extracellular matrix scaffolds.Acta biomaterialia · 2018Article
- Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments.Journal of cardiothoracic surgery · 2018Article
- Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial.Immunologic research · 2018Article
- Magnesium Presence Prevents Removal of Antigenic Nuclear-Associated Proteins from Bovine Pericardium for Heart Valve Engineering.Tissue engineering. Part A · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Bovine pericardium is an important biomaterial with current application in glutaraldehyde-fixed bioprosthetic heart valves and possible future application as an unfixed biological scaffold for tissue engineering. The importance of both humoral and cell-mediated rejection responses toward fixed and unfixed xenogeneic tissues has become increasingly apparent. However, the full scope and specific identities of bovine pericardium proteins that can elicit an immune response remain largely unknown. In this study, an immunoproteomic approach was used to survey bovine pericardium proteins for their ability to elicit a humoral immune response in rabbits. A two-stage protein extraction protocol was used to separate bovine pericardium proteins into water- and lipid-soluble fractions. Two-dimensional (2-D) gel electrophoresis was performed to separate the proteins from each fraction. Western blots were generated from 2-D gels of both bovine pericardium protein fractions. These blots were probed with serum from rabbits immunized with bovine pericardium and a secondary antibody was used to assess for IgG positivity. Western blots were compared to duplicate 2-D gels and proteins in matched spots were identified by tandem mass spectrometry. Thirty-one putative protein antigens were identified, eight of which are known to be antigenic from previous studies. All of the putative antigens demonstrated progressive staining intensity with increasing days of post-exposure serum. Identified antigenic proteins represented a variety of functional and structural protein types, and included both cellular and matrix proteins. The results of this study have implications for the use of bovine pericardium as a biomaterial in bioprostheses and tissue engineering applications, as well as xenotransplantation in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.