ArticleJournal of leukocyte biology2008
Separate endocytic pathways regulate IL-5 receptor internalization and signaling.
Article in Journal of leukocyte biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis.Frontiers in immunology · 2024Article
- Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells.Biophysical reports · 2022Article
- Extracellular matrix promotes clathrin-dependent endocytosis of prolactin and STAT5 activation in differentiating mammary epithelial cells.Scientific reports · 2017Article
- Cytokine Receptor Endocytosis: New Kinase Activity-Dependent and -Independent Roles of PI3K.Frontiers in endocrinology · 2017Review
- Glycobiology of Eosinophilic Inflammation: Contributions of Siglecs, Glycans, and Other Glycan-Binding Proteins.Frontiers in medicine · 2017Review
- Transcription Factor Repertoire of Homeostatic Eosinophilopoiesis.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Cholesterol selectively regulates IL-5 induced mitogen activated protein kinase signaling in human eosinophils.PloS one · 2014Article
- Autophagy regulates phagocytosis by modulating the expression of scavenger receptors.Immunity · 2013Article
- Quantifying crosstalk among interferon-γ, interleukin-12, and tumor necrosis factor signaling pathways within a TH1 cell model.Science signaling · 2012Article
- Clathrin mediates endocytosis of progastrin and activates MAPKs: role of cell surface annexin A2.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Dynamic ligand modulation of EPO receptor pools, and dysregulation by polycythemia-associated EPOR alleles.PloS one · 2012Article
- The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice.PloS one · 2012Article
- Constitutive exposure of phosphatidylserine on viable cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Three lysine residues in the common β chain of the interleukin-5 receptor are required for Janus kinase (JAK)-dependent receptor ubiquitination, endocytosis, and signaling.The Journal of biological chemistry · 2011Article
- Cytokine signaling modulates blood-brain barrier function.Current pharmaceutical design · 2011Review
- IL-5 receptor α levels in patients with marked eosinophilia or mastocytosis.The Journal of allergy and clinical immunology · 2011Article
- T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Inferring relevant control mechanisms for interleukin-12 signaling in naïve CD4+ T cells.Immunology and cell biology · 2011Article
- An empirical Bayesian approach for model-based inference of cellular signaling networks.BMC bioinformatics · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Eosinophils are critically dependent on IL-5 for their activation, differentiation, survival, and augmentation of cytotoxic activity. We previously showed that the cytoplasmic domain of the hematopoietic receptor, betac, which is shared by IL-5, IL-3, and GM-CSF, is directly ubiquitinated and degraded by the proteasomes in a JAK2-dependent manner. However, studies describing the spatial distribution, endocytic regulation, and trafficking of betac-sharing receptors in human eosinophils are currently lacking. Using deconvolution microscopy and biochemical methods, we clearly demonstrate that IL-5Rs reside in and are internalized by clathrin- and lipid raft-dependent endocytic pathways. Microscopy analyses in TF1 cells and human eosinophils revealed significant colocalization of betac, IL-5Ralpha, and Cy3-labeled IL-5 with transferrin- (clathrin) and cholera toxin-B- (lipid raft) positive vesicles. Moreover, whereas internalized IL-5Rs were detected in both clathrin- and lipid raft-positive vesicles, biochemical data revealed that tyrosine phosphorylated, ubiquitinated, and proteasome-degraded IL-5Rs partitioned to the soluble, nonraft fractions (clathrin-containing). Lastly, we show that optimal IL-5-induced signaling requires entry of activated IL-5Rs into the intracellular compartment, as coimmunoprecipitation of key signaling molecules with the IL-5R was completely blocked when either endocytic pathway was inhibited. These data provide the first evidence that IL-5Rs segregate and traffic into two distinct plasma membrane compartments, and they further establish that IL-5R endocytosis regulates signaling both positively and negatively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.