ArticleMolecular endocrinology (Baltimore, Md.)2008
The Xenopus laevis isoform of G protein-coupled receptor 3 (GPR3) is a constitutively active cell surface receptor that participates in maintaining meiotic arrest in X. laevis oocytes.
Article in Molecular endocrinology (Baltimore, Md.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- GPR3 in neuro-metabolic-immune-reproductive nexus - a potential therapeutic target for Multi-System diseases.Annals of medicine · 2026Pooled it
- Discovery of Small Molecule Ligands Targeting Orphan G Protein-Coupled Receptors GPR3, GPR6, and GPR12.Journal of medicinal chemistry · 2026Review
- Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics.eLife · 2025Article
- Rare variants in GPR3 in POI patients: a case series with review of literature.Journal of ovarian research · 2023Review
- Regulation of oocyte maturation: Role of conserved ERK signaling.Molecular reproduction and development · 2022Review
- Oocyte maturation abnormalities - A systematic review of the evidence and mechanisms in a rare but difficult to manage fertility pheneomina.Turkish journal of obstetrics and gynecology · 2022Article
- Silencing the G-protein coupled receptor 3-salt inducible kinase 2 pathway promotes human β cell proliferation.Communications biology · 2021Article
- Article
- Review
- Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile.International journal of molecular sciences · 2020Article
- Dissection of the Ovulatory Process UsingFrontiers in cell and developmental biology · 2020Review
- GPR3, GPR6, and GPR12 as novel molecular targets: their biological functions and interaction with cannabidiol.Acta pharmacologica Sinica · 2019Review
- The art of oocyte meiotic arrest regulation.Reproductive biology and endocrinology : RB&E · 2019Review
- Phosphorylation Dynamics Dominate the Regulated Proteome during Early Xenopus Development.Scientific reports · 2017Article
- Paxillin and embryonic PolyAdenylation Binding Protein (ePABP) engage to regulate androgen-dependent Xenopus laevis oocyte maturation - A model of kinase-dependent regulation of protein expression.Molecular and cellular endocrinology · 2017Article
- Mice lacking GPR3 receptors display late-onset obese phenotype due to impaired thermogenic function in brown adipose tissue.Scientific reports · 2015Article
- Vertebrate Reproduction.Cold Spring Harbor perspectives in biology · 2015Review
- Overlapping nongenomic and genomic actions of thyroid hormone and steroids.Best practice & research. Clinical endocrinology & metabolism · 2015Review
- Paxillin and steroid signaling: from frog to human.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Regulation of Constitutive GPR3 Signaling and Surface Localization by GRK2 and β-arrestin-2 Overexpression in HEK293 Cells.PloS one · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Oocytes are held in meiotic arrest in prophase I until ovulation, when gonadotropins trigger a subpopulation of oocytes to resume meiosis in a process termed "maturation." Meiotic arrest is maintained through a mechanism whereby constitutive cAMP production exceeds phosphodiesterase-mediated degradation, leading to elevated intracellular cAMP. Studies have implicated a constitutively activated Galpha(s)-coupled receptor, G protein-coupled receptor 3 (GPR3), as one of the molecules responsible for maintaining meiotic arrest in mouse oocytes. Here we characterized the signaling and functional properties of GPR3 using the more amenable model system of Xenopus laevis oocytes. We cloned the X. laevis isoform of GPR3 (XGPR3) from oocytes and showed that overexpressed XGPR3 elevated intraoocyte cAMP, in large part via Gbetagamma signaling. Overexpressed XGPR3 suppressed steroid-triggered kinase activation and maturation of isolated oocytes, as well as gonadotropin-induced maturation of follicle-enclosed oocytes. In contrast, depletion of XGPR3 using antisense oligodeoxynucleotides reduced intracellular cAMP levels and enhanced steroid- and gonadotropin-mediated oocyte maturation. Interestingly, collagenase treatment of Xenopus oocytes cleaved and inactivated cell surface XGPR3, which enhanced steroid-triggered oocyte maturation and activation of MAPK. In addition, human chorionic gonadotropin-treatment of follicle-enclosed oocytes triggered metalloproteinase-mediated cleavage of XGPR3 at the oocyte cell surface. Together, these results suggest that GPR3 moderates the oocyte response to maturation-promoting signals, and that gonadotropin-mediated activation of metalloproteinases may play a partial role in sensitizing oocytes for maturation by inactivating constitutive GPR3 signaling.
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