ArticleBMC genomics2008
Genome-wide gene expression profiling analysis of Leishmania major and Leishmania infantum developmental stages reveals substantial differences between the two species.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 144 citations in OpenAlex.
- Single-cell atlas ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
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- Quantitative single-cell analysis of Leishmania major amastigote differentiation demonstrates variably extended expression of the lipophosphoglycan (LPG) virulence factor in different host cell types.PLoS neglected tropical diseases · 2022Article
- In vivo transcriptional analysis of mice infected with Leishmania major unveils cellular heterogeneity and altered transcriptomic profiling at single-cell resolution.PLoS neglected tropical diseases · 2022Article
- Tissue Specific Dual RNA-Seq Defines Host-Parasite Interplay in Murine Visceral Leishmaniasis Caused by Leishmania donovani and Leishmania infantum.Microbiology spectrum · 2022Article
- Comparative analysis of the transcriptional responses of five Leishmania species to trivalent antimony.Parasites & vectors · 2021Article
- TheGenes · 2021Article
- Stage-Specific Differential Gene Expression of Glutathione Peroxidase inReports of biochemistry & molecular biology · 2020Article
- An immunoinformatic approach driven by experimental proteomics: in silico design of a subunit candidate vaccine targeting secretory proteins of Leishmania donovani amastigotes.Parasites & vectors · 2020Article
- Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major.Scientific reports · 2019Article
- Comparative transcriptomics in Leishmania braziliensis: disclosing differential gene expression of coding and putative noncoding RNAs across developmental stages.RNA biology · 2019Article
- P-type transport ATPases in Leishmania and Trypanosoma.Parasite (Paris, France) · 2019Review
- Pathogen Evasion of Chemokine Response Through Suppression of CXCL10.Frontiers in cellular and infection microbiology · 2019Article
- Development of an automated image analysis protocol for quantification of intracellular forms of Leishmania spp.PloS one · 2018Article
- CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1.PloS one · 2018Article
- The Pumilio-domain protein PUF6 contributes to SIDER2 retroposon-mediated mRNA decay inRNA (New York, N.Y.) · 2017Article
- Transcriptome profiling identifies genes/pathways associated with experimental resistance to paromomycin in Leishmania donovani.International journal for parasitology. Drugs and drug resistance · 2017Article
- Establishing multiple omics baselines for three Southeast Asian populations in the Singapore Integrative Omics Study.Nature communications · 2017Article
- Gene Expression inmBio · 2017Article
- Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmania parasites cause a diverse spectrum of diseases in humans ranging from spontaneously healing skin lesions (e.g., L. major) to life-threatening visceral diseases (e.g., L. infantum). The high conservation in gene content and genome organization between Leishmania major and Leishmania infantum contrasts their distinct pathophysiologies, suggesting that highly regulated hierarchical and temporal changes in gene expression may be involved.
resultsWe used a multispecies DNA oligonucleotide microarray to compare whole-genome expression patterns of promastigote (sandfly vector) and amastigote (mammalian macrophages) developmental stages between L. major and L. infantum. Seven per cent of the total L. infantum genome and 9.3% of the L. major genome were differentially expressed at the RNA level throughout development. The main variations were found in genes involved in metabolism, cellular organization and biogenesis, transport and genes encoding unknown function. Remarkably, this comparative global interspecies analysis demonstrated that only 10-12% of the differentially expressed genes were common to L. major and L. infantum. Differentially expressed genes are randomly distributed across chromosomes further supporting a posttranscriptional control, which is likely to involve a variety of 3'UTR elements.
conclusionThis study highlighted substantial differences in gene expression patterns between L. major and L. infantum. These important species-specific differences in stage-regulated gene expression may contribute to the disease tropism that distinguishes L. major from L. infantum.
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