ArticleJournal of anatomy2008
Quantification of diverse subcellular immunohistochemical markers with clinicobiological relevancies: validation of a new computer-assisted image analysis procedure.
Article in Journal of anatomy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Longitudinal evaluation of tumor-infiltrating lymphocyte scoring using automated region of interest registration in breast cancer.Breast cancer research : BCR · 2026Article
- Intratumoral Heterogeneity of Ki67 Index and Its Impact on the Diagnosis and Prognostication of Pancreatic Neuroendocrine Tumors: Hot-Spot Count Differs Significantly from Random-Area Count.Endocrine pathology · 2025Article
- Mismatch Repair Proteins Immunostaining in Lip Squamous Cell Carcinoma: A Role in Lip Carcinogenesis?Asian Pacific journal of cancer prevention : APJCP · 2025Article
- Article
- Strobilanthes crispus elicits anti-tumor immunogenicity in in vitro and in vivo metastatic breast carcinoma.PloS one · 2022Article
- How the variability between computer-assisted analysis procedures evaluating immune markers can influence patients' outcome prediction.Histochemistry and cell biology · 2021Article
- Developmental Programming: Prenatal Testosterone Excess on Ovarian SF1/DAX1/FOXO3.Reproductive sciences (Thousand Oaks, Calif.) · 2020Article
- Prenatal Testosterone Excess Disrupts Placental Function in a Sheep Model of Polycystic Ovary Syndrome.Endocrinology · 2019Article
- Glia to neuron ratio in the posterior aspect of the human spinal cord at thoracic segments relevant to spinal cord stimulation.Journal of anatomy · 2019Article
- Computer-assisted image analysis of the tumor microenvironment on an oral tongue squamous cell carcinoma tissue microarray.Clinical and translational radiation oncology · 2019Article
- DeepQuantify: deep learning and quantification system of white blood cells in light microscopy images of injured skeletal muscles.Journal of medical imaging (Bellingham, Wash.) · 2019Article
- Developmental programming: Changes in mediators of insulin sensitivity in prenatal bisphenol A-treated female sheep.Reproductive toxicology (Elmsford, N.Y.) · 2019Article
- Translational AI and Deep Learning in Diagnostic Pathology.Frontiers in medicine · 2019Review
- Developmental Programming: Gestational Exposure to Excess Testosterone Alters Expression of Ovarian Matrix Metalloproteases and Their Target Proteins.Reproductive sciences (Thousand Oaks, Calif.) · 2018Article
- Developmental Programming: Impact of Gestational Steroid and Metabolic Milieus on Mediators of Insulin Sensitivity in Prenatal Testosterone-Treated Female Sheep.Endocrinology · 2017Article
- Phosphodiesterases in non-neoplastic appearing colonic mucosa from patients with colorectal neoplasia.BMC cancer · 2016Article
- Evaluation of cytokeratin-19 in breast cancer tissue samples: a comparison of automatic and manual evaluations of scanned tissue microarray cylinders.Biomedical engineering online · 2015Article
- Automated analysis of immunohistochemistry images identifies candidate location biomarkers for cancers.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Effects of tissue decalcification on the quantification of breast cancer biomarkers by digital image analysis.Diagnostic pathology · 2014Article
- Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue microarray technology and immunohistochemical techniques have become a routine and indispensable tool for current anatomical pathology diagnosis. However, manual quantification by eye is relatively slow and subjective, and the use of digital image analysis software to extract information of immunostained specimens is an area of ongoing research, especially when the immunohistochemical signals have different localization in the cells (nuclear, membrane, cytoplasm). To minimize critical aspects of manual quantitative data acquisition, we generated semi-automated image-processing steps for the quantification of individual stained cells with immunohistochemical staining of different subcellular location. The precision of these macros was evaluated in 196 digital colour images of different Hodgkin lymphoma biopsies stained for different nuclear (Ki67, p53), cytoplasmic (TIA-1, CD68) and membrane markers (CD4, CD8, CD56, HLA-Dr). Semi-automated counts were compared to those obtained manually by three separate observers. Paired t-tests demonstrated significant differences between intra- and inter-observer measurements, with more substantial variability when the cellular density of the digital images was > 100 positive cells/image. Overall, variability was more pronounced for intra-observer than for inter-observer comparisons, especially for cytoplasmic and membrane staining patterns (P < 0.0001 and P = 0.050). The comparison between the semi-automated and manual microscopic measurement methods indicates significantly lower variability in the results yielded by the former method. Our semi-automated computerized method eliminates the major causes of observer variability and may be considered a valid alternative to manual microscopic quantification for diagnostic, prognostic and therapeutic purposes.
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