ArticleNanomedicine (London, England)2008
Detection of HIV-1 p24 Gag in plasma by a nanoparticle-based bio-barcode-amplification method.
Article in Nanomedicine (London, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Sigma-1 receptor regulates HIV-1 and methamphetamine-induced endothelial/pericyte barrier impairment via strain-specific inflammatory responses and mitochondrial dysregulation.Journal of neuroinflammation · 2026Article
- Combining Bioorthogonal Chemistry with Fluorescent Silica Nanoparticles for the Ultrasensitive Detection of the HIV-1 p24 Antigen.ACS omega · 2024Article
- Diagnosis of genitourinary tuberculosis: detection of mycobacterial lipoarabinomannan and MPT-64 biomarkers within urine extracellular vesicles by nano-based immuno-PCR assay.Scientific reports · 2023Article
- Nanoscale dynamic chemical, biological sensor material designs for control monitoring and early detection of advanced diseases.Materials today. Bio · 2020Review
- Materials Science in the time of Coronavirus.Journal of materials science · 2020Article
- Bio-barcode detection technology and its research applications: A review.Journal of advanced research · 2019Review
- Biomedical applications of nanoflares: Targeted intracellular fluorescence probes.Nanomedicine : nanotechnology, biology, and medicine · 2019Review
- Applications of gold nanoparticles in virus detection.Theranostics · 2018Review
- Detection ofInternational journal of nanomedicine · 2018Article
- Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections.Nanomedicine : nanotechnology, biology, and medicine · 2017Review
- Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence.Chemical reviews · 2015Review
- Increased Sensitivity of HIV-1 p24 ELISA Using a Photochemical Signal Amplification System.Journal of acquired immune deficiency syndromes (1999) · 2015Article
- Direct Reading of Bona Fide Barcode Assays for Diagnostics with Smartphone Apps.Scientific reports · 2015Article
- Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering.Nature nanotechnology · 2014Review
- Virus detection and quantification using electrical parameters.Scientific reports · 2014Article
- Post-genomics nanotechnology is gaining momentum: nanoproteomics and applications in life sciences.Omics : a journal of integrative biology · 2014Review
- Preclinical detection of porcine circovirus type 2 infection using an ultrasensitive nanoparticle DNA probe-based PCR assay.PloS one · 2014Article
- A liposome-based ion release impedance sensor for biological detection.Biomedical microdevices · 2013Article
- Applications of gold nanoparticles in the detection and identification of infectious diseases and biothreats.Advanced materials (Deerfield Beach, Fla.) · 2013Article
- Two types of nanoparticle-based bio-barcode amplification assays to detect HIV-1 p24 antigen.Virology journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundDetection of HIV-1 in patients is limited by the sensitivity and selectivity of available tests. The nanotechnology-based bio-barcode-amplification method offers an innovative approach to detect specific HIV-1 antigens from diverse HIV-1 subtypes. We evaluated the efficacy of this protein-detection method in detecting HIV-1 in men enrolled in the Chicago component of the Multicenter AIDS Cohort Study (MACS).
methodsThe method relies on magnetic microparticles with antibodies that specifically bind the HIV-1 p24 Gag protein and nanoparticles that are encoded with DNA and antibodies that can sandwich the target protein captured by the microparticle-bound antibodies. The aggregate sandwich structures are magnetically separated from solution, and treated to remove the conjugated barcode DNA. The DNA barcodes (hundreds per target) were identified by a nanoparticle-based detection method that does not rely on PCR.
resultsOf 112 plasma samples from HIV-1-infected subjects, 111 were positive for HIV-1 p24 Gag protein (range: 0.11-71.5 ng/ml of plasma) by the bio-barcode-amplification method. HIV-1 p24 Gag protein was detected in only 23 out of 112 men by the conventional ELISA. A total of 34 uninfected subjects were negative by both tests. Thus, the specificity of the bio-barcode-amplification method was 100% and the sensitivity 99%. The bio-barcode-amplification method detected HIV-1 p24 Gag protein in plasma from all study subjects with less than 200 CD4(+) T cells/microl of plasma (100%) and 19 out of 20 (95%) HIV-1-infected men who had less than 50 copies/ml of plasma of HIV-1 RNA. In a separate group of 60 diverse international isolates, representative of clades A, B, C and D and circulating recombinant forms CRF01_AE and CRF02_AG, the bio-barcode-amplification method identified the presence of virus correctly.
conclusionsThe bio-barcode-amplification method was superior to the conventional ELISA assay for the detection of HIV-1 p24 Gag protein in plasma with a breadth of coverage for diverse HIV-1 subtypes. Because the bio-barcode-amplification method does not require enzymatic amplification, this method could be translated into a robust point-of-care test.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.