ArticleLangmuir : the ACS journal of surfaces and colloids2008
Double cushions preserve transmembrane protein mobility in supported bilayer systems.
Article in Langmuir : the ACS journal of surfaces and colloids, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 105 citations in OpenAlex.
- Tutorial: biomembranes in hybrid living bioelectronics.Nature protocols · 2026Review
- Lipid-Substrate Interactions Lead to Bilayer Asymmetry.Journal of the American Chemical Society · 2026Article
- A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Leaflet-Specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers.Angewandte Chemie (International ed. in English) · 2025Article
- Binding ofLangmuir : the ACS journal of surfaces and colloids · 2024Article
- Phosphatidic Acid Accumulates at Areas of Curvature in Tubulated Lipid Bilayers and Liposomes.Biomolecules · 2022Article
- Fast slow folding of an outer membrane porin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Advances in Cell-Conductive Polymer Biointerfaces and Role of the Plasma Membrane.Chemical reviews · 2022Article
- Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers.Biophysical journal · 2021Article
- Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications.Polymers · 2020Review
- Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Spherical-supported membranes as platforms for screening against membrane protein targets.Analytical biochemistry · 2018Article
- Supported lipid bilayer platforms to probe cell mechanobiology.Biochimica et biophysica acta. Biomembranes · 2017Review
- Multiplexed Lipid Bilayers on Silica Microspheres for Analytical Screening Applications.Analytical chemistry · 2017Article
- Viral fusion efficacy of specific H3N2 influenza virus reassortant combinations at single-particle level.Scientific reports · 2016Article
- Electronic control of HNature communications · 2016Article
- SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Photopolymerization of Dienoyl Lipids Creates Planar Supported Poly(lipid) Membranes with Retained Fluidity.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Reconstitution of proteins on electroformed giant unilamellar vesicles.Methods in cell biology · 2015Article
- Transmembrane protein diffusion in gel-supported dual-leaflet membranes.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Supported lipid bilayers (SLBs) have been widely used as model systems to study cell membrane processes because they preserve the same 2D membrane fluidity found in living cells. One of the most significant limitations of this platform, however, is its inability to incorporate mobile transmembrane species. It is often postulated that transmembrane proteins reconstituted in SLBs lose their mobility because of direct interactions between the protein and the underlying substrate. Herein, we demonstrate a highly mobile fraction for a transmembrane protein, annexin V. Our strategy involves supporting the lipid bilayer on a double cushion, where we not only create a large space to accommodate the transmembrane portion of the macromolecule but also passivate the underlying substrate to reduce nonspecific protein-substrate interactions. The thickness of the confined water layer can be tuned by fusing vesicles containing polyethyleneglycol (PEG)-conjugated lipids of various molecular weights to a glass substrate that has first been passivated with a sacrificial layer of bovine serum albumin (BSA). The 2D fluidity of these systems was characterized by fluorescence recovery after photobleaching (FRAP) measurements. Uniform, mobile phospholipid bilayers with lipid diffusion coefficients of around 3 x 10(-8) cm2/s and percent mobile fractions of over 95% were obtained. Moreover, we obtained annexin V diffusion coefficients that were also around 3 x 10(-8) cm2/s with mobile fractions of up to 75%. This represents a significant improvement over bilayer platforms fabricated directly on glass or using single cushion strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.