ArticleJournal of molecular neuroscience : MN2008
Identification of intracellular signaling cascades mediating the PACAP-induced increase in guinea pig cardiac neuron excitability.
Article in Journal of molecular neuroscience : MN, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- β-arrestin1 orchestrates endosomal signaling to regulate translational control of circadian light entrainment.Communications biology · 2026Article
- Src family kinase inhibitors blunt PACAP-induced PAC1 receptor endocytosis, phosphorylation of ERK, and the increase in cardiac neuron excitability.American journal of physiology. Cell physiology · 2018Article
- PACAP signaling in stress: insights from the chromaffin cell.Pflugers Archiv : European journal of physiology · 2018Review
- Recruitment of endosomal signaling mediates the forskolin modulation of guinea pig cardiac neuron excitability.American journal of physiology. Cell physiology · 2017Article
- Activation of MEK/ERK signaling contributes to the PACAP-induced increase in guinea pig cardiac neuron excitability.American journal of physiology. Cell physiology · 2016Article
- Activation of MEK/ERK Signaling by PACAP in Guinea Pig Cardiac Neurons.Journal of molecular neuroscience : MN · 2016Article
- Nickel suppresses the PACAP-induced increase in guinea pig cardiac neuron excitability.American journal of physiology. Cell physiology · 2015Article
- Pituitary adenylate cyclase 1 receptor internalization and endosomal signaling mediate the pituitary adenylate cyclase activating polypeptide-induced increase in guinea pig cardiac neuron excitability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Pretreatment with nonselective cationic channel inhibitors blunts the PACAP-induced increase in guinea pig cardiac neuron excitability.Journal of molecular neuroscience : MN · 2012Article
- VIP and PACAP effects on mouse major pelvic ganglia neurons.Journal of molecular neuroscience : MN · 2010Article
- Protective effect of PACAP against doxorubicin-induced cell death in cardiomyocyte culture.Journal of molecular neuroscience : MN · 2010Article
- Remodeling of the guinea pig intrinsic cardiac plexus with chronic pressure overload.American journal of physiology. Regulatory, integrative and comparative physiology · 2009Article
- Enhancement of Ih, but not inhibition of IM, is a key mechanism underlying the PACAP-induced increase in excitability of guinea pig intrinsic cardiac neurons.American journal of physiology. Regulatory, integrative and comparative physiology · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The pituitary adenylate cyclase-activating polypeptide (PACAP) increases excitability of guinea pig cardiac neurons, an effect mediated through activation of PAC1 receptors. The signaling cascades that couple activation of the PAC1 receptor to alterations in membrane ionic conductances responsible for the PACAP effect are unknown. Intracellular recordings were made from neurons in kinase inhibitor-treated cardiac ganglia preparations to determine which of the intracellular cascades activated by PAC1 receptor stimulation mediate the PACAP effect. In control cells, long depolarizing-current steps elicited one to three action potentials. In contrast, during the application of 10 nM PACAP, depolarizing-current pulses elicited multiple action potential firing (greater than or equal to five action potentials) in 79% of the neurons. Pretreatment with an adenylyl cyclase inhibitor, SQ 22536 (100 microM), suppressed the PACAP-induced increase in excitability, whereas the presence of U-73122 (10 microM), a potent phospholipase C (PLC) inhibitor, had no effect. Thus, the activation of adenylyl cyclase, but not PLC, was a critical step mediating the PACAP effect. Pretreatment with H-89 (1 microM), a protein kinase A inhibitor, and PD 98059 (50 microM), a MEK kinase inhibitor, also significantly blunted the PACAP-induced increase in excitability. Furthermore, treatment with forskolin (5 microM), an activator of adenylyl cyclase, or exposure to the cell-permeable cyclic adenosine monophosphate (cAMP) analogue, 8-bromo-cAMP (1 mM), partially recapitulated the effect of PACAP on excitability. We conclude that the activation of signaling cascades downstream of cAMP mediate the PACAP-induced increase in cardiac neuron excitability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.