ArticlePloS one2008
Activation of estrogen receptor-alpha by E2 or EGF induces temporally distinct patterns of large-scale chromatin modification and mRNA transcription.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- Sensitive image-based chromatin binding assays using inducible ERα to rapidly characterize estrogenic chemicals and mixtures.iScience · 2022Article
- A Mechanistic High-Content Analysis Assay Using a Chimeric Androgen Receptor That Rapidly Characterizes Androgenic Chemicals.SLAS discovery : advancing life sciences R & D · 2020Article
- Steroid hormones regulate genome-wide epigenetic programming and gene transcription in human endometrial cells with marked aberrancies in endometriosis.PLoS genetics · 2020Article
- Characterizing properties of non-estrogenic substituted bisphenol analogs using high throughput microscopy and image analysis.PloS one · 2017Article
- Role of Estrogen Response Element in the Human Prolactin Gene: Transcriptional Response and Timing.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Differential Regulation of Progesterone Receptor-Mediated Transcription by CDK2 and DNA-PK.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Minireview: Tipping the balance: ligand-independent activation of steroid receptors.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Article
- Estrogen receptor alpha prevents bladder cancer via INPP4B inhibited akt pathway in vitro and in vivo.Oncotarget · 2014Article
- Dynamic regulation of transcriptional states by chromatin and transcription factors.Nature reviews. Genetics · 2014Review
- Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.Nucleic acids research · 2013Article
- The prolactin gene: a paradigm of tissue-specific gene regulation with complex temporal transcription dynamics.Journal of neuroendocrinology · 2012Review
- Expression patterns of ER, HER2, and NM23-H1 in breast cancer patients with different menopausal status: correlations with metastasis.Molecular diagnosis & therapy · 2011Article
- Article
- Automated microscopy and image analysis for androgen receptor function.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Growth factor-induced resistance to tamoxifen is associated with a mutation of estrogen receptor alpha and its phosphorylation at serine 305.Breast cancer research and treatment · 2010Article
- Article
- Cellular targets of estrogen signaling in regeneration of inner ear sensory epithelia.Hearing research · 2009Review
- The myImageAnalysis project: a web-based application for high-content screening.Assay and drug development technologiesArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Estrogen receptor-alpha (ER) transcription function is regulated in a ligand-dependent (e.g., estradiol, E2) or ligand-independent (e.g., growth factors) manner. Our laboratory seeks to understand these two modes of action. Using a cell line that contains a visible prolactin enhancer/promoter array (PRL-HeLa) regulated by ER, we analyzed ER response to E2 and EGF by quantifying image-based results. Data show differential recruitment of GFP-ER to the array, with the AF1 domain playing a vital role in EGF-mediated responsiveness. Temporal analyses of large-scale chromatin dynamics, and accumulation of array-localized reporter mRNA over 24 hours showed that the EGF response consists of a single pulse of reporter mRNA accumulation concomitant with transient increase in array decondensation. Estradiol induced a novel cyclical pattern of mRNA accumulation with a sustained increase in array decondensation. Collectively, our work shows that there is a stimuli-specific pattern of large-scale chromatin modification and transcript levels by ER.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.