ArticlePlant physiology2008
Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 189 citations in OpenAlex.
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Three-dimensional visualization of tip-vesicles in growing pollen tubes by electron tomography.Science China. Life sciences · 2026Article
- Female gametophyte development, pollen‒pistil interactions and embryogenic patterns in chicory (Cichorium intybus): a self-incompatibility perspective.Plant cell reports · 2025Article
- Analytical methods for cytoplasmic streaming in elongated cells.PNAS nexus · 2025Article
- Conserved physical mechanisms of cell and tissue elongation.Development (Cambridge, England) · 2024Review
- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Arabidopsis Pollen Tubes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- VAMP726 and VAMP725 regulate vesicle secretion and pollen tube growth in Arabidopsis.Plant cell reports · 2023Article
- Genetic Regulation of Mitosis-Meiosis Fate Decision in Plants: Is Callose an Oversighted Polysaccharide in These Processes?Plants (Basel, Switzerland) · 2023Review
- Plant cell-like tip-growing polymer precipitate with structurally embedded multistimuli sensing ability.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- Asymmetric distribution of extracellular matrix proteins in seed coat epidermal cells of Arabidopsis is determined by polar secretion.Plant direct · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Membrane imaging in the plant endomembrane system.Plant physiology · 2021Article
- The quest for the central players governing pollen tube growth and guidance.Plant physiology · 2021Review
- LC-MS based metabolic fingerprinting of apricot pistils after self-compatible and self-incompatible pollinations.Plant molecular biology · 2021Article
- Rab-E and its interaction with myosin XI are essential for polarised cell growth.The New phytologist · 2021Article
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O-arabinosyltransferase mutants.The Plant journal : for cell and molecular biology · 2020Article
- For things to stay the same, things must change: polyploidy and pollen tube growth rates.Annals of botany · 2020Article
- Exocytosis and endocytosis: coordinating and fine-tuning the polar tip growth domain in pollen tubes.Journal of experimental botany · 2020Review
33 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The delivery of cell wall material and membrane to growing plant cell surfaces requires the spatial and temporal coordination of secretory vesicle trafficking. Given the small size of vesicles, their dynamics is difficult to quantify. To quantitatively analyze vesicle dynamics in growing pollen tubes labeled with the styryl dye FM1-43, we applied spatiotemporal correlation spectroscopy on time-lapse series obtained with high-speed confocal laser scanning microscopy recordings. The resulting vector maps revealed that vesicles migrate toward the apex in the cell cortex and that they accumulate in an annulus-shaped region adjacent to the extreme tip and then turn back to flow rearward in the center of the tube. Fluorescence recovery after photobleaching confirmed vesicle accumulation in the shoulder of the apex, and it revealed that the extreme apex never recovers full fluorescence intensity. This is consistent with endocytotic activity occurring in this region. Fluorescence recovery after photobleaching analysis also allowed us to measure the turnover rate of the apical vesicle population, which was significantly more rapid than the theoretical rate computed based on requirements for new cell wall material. This may indicate that a significant portion of the vesicles delivered to the apex does not succeed in contacting the plasma membrane for delivery of their contents. Therefore, we propose that more than one passage into the apex may be needed for many vesicles before they fuse to the plasma membrane and deliver their contents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.