ArticleJournal of clinical microbiology2008
Multiplex real-time PCR assay for rapid identification of Mycobacterium tuberculosis complex members to the species level.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 78 citations in OpenAlex.
- Biographical Feature: Benjamin A. Pinsky, MD, PhD.Journal of clinical microbiology · 2026Article
- Virulence ofVeterinary medicine international · 2026Article
- Development and evaluation of a triplex droplet digital PCR method for differentiation ofFrontiers in microbiology · 2024Article
- The effectiveness of bovine tuberculosis surveillance in Dutch badgers.Transboundary and emerging diseases · 2022Article
- Rapid Identification ofFrontiers in microbiology · 2022Article
- Development of a Multiplex Real-Time PCR Assay for Mycobacterium bovis BCG and Validation in a Clinical Laboratory.Microbiology spectrum · 2021Article
- A solitary pulmonary nodule caused by Mycobacterium tuberculosis var. BCG after intravesical BCG treatment: a case report.BMC pulmonary medicine · 2021Article
- Accurate Identification of Closely RelatedFrontiers in cellular and infection microbiology · 2021Article
- Three-reaction high-resolution melting assay for rapid differentiation of Mycobacterium tuberculosis complex members.MicrobiologyOpen · 2019Article
- Development of a new High Resolution Melting (HRM) assay for identification and differentiation of Mycobacterium tuberculosis complex samples.Scientific reports · 2019Article
- Practical Guidance for Clinical Microbiology Laboratories: Mycobacteria.Clinical microbiology reviews · 2018Review
- First-time detection and identification of the Mycobacterium tuberculosis Complex members in extrapulmonary tuberculosis clinical samples in south Tunisia by a single tube tetraplex real-time PCR assay.PLoS neglected tropical diseases · 2017Article
- Article
- Antimycobacterial activity of Pichia pastoris-derived mature bovine neutrophil β-defensins 5.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Evaluation of molecular markers for the diagnosis of Mycobacterium bovis.Folia microbiologica · 2014Article
- An efficient alternative marker for specific identification of Mycobacterium tuberculosis.World journal of microbiology & biotechnology · 2014Article
- Standing of nucleic acid testing strategies in veterinary diagnosis laboratories to uncover Mycobacterium tuberculosis complex members.Frontiers in molecular biosciences · 2014Review
- A simple and efficient multiplex PCR assay for the identification of Mycobacterium genus and Mycobacterium tuberculosis complex to the species level.Yonsei medical journal · 2013Article
- Region of difference 4 in alpine Mycobacterium caprae isolates indicates three variants.Journal of clinical microbiology · 2013Article
- Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon.Tropical animal health and production · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The species identification of members of the Mycobacterium tuberculosis complex is critical to the timely initiation of both appropriate antibiotic therapy and proper public health control measures. However, the current commercially available molecular assays identify mycobacteria only to the complex level and are unable to differentiate M. tuberculosis from the closely related M. bovis and M. bovis BCG. We describe here a rapid and robust two-step, multiplex, real-time PCR assay based on genomic deletions to definitively identify M. tuberculosis, M. bovis, M. bovis BCG, and other members of the complex. When tested against a panel of well-characterized mycobacterial reference strains, the assay was both sensitive and specific, correctly identifying all strains. We applied this assay to 60 clinical isolates previously identified as M. tuberculosis complex and found 57 M. tuberculosis isolates and 3 M. bovis BCG isolates from patients who had received intravesical BCG. Furthermore, analysis of 15 clinical specimens previously identified as M. bovis by spoligotyping revealed an isolate of M. tuberculosis that had been misidentified. We propose that this assay will allow the routine identification of M. tuberculosis complex members in the clinical laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.