ArticleMolecular biology of the cell2008
Xenopus TACC3/maskin is not required for microtubule stability but is required for anchoring microtubules at the centrosome.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanistic design of cell-penetrating disruptors for phospho-dependent TACC3-CHC interaction.Structure (London, England : 1993) · 2026Article
- Aurora A site specific TACC3 phosphorylation regulates astral microtubule assembly by stabilizing γ-tubulin ring complex.BMC molecular and cell biology · 2019Article
- Wolf-Hirschhorn Syndrome-Associated Genes Are Enriched in Motile Neural Crest Cells and Affect Craniofacial Development inFrontiers in physiology · 2019Article
- Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation.Science (New York, N.Y.) · 2018Article
- Structural analysis of the role of TPX2 in branching microtubule nucleation.The Journal of cell biology · 2017Article
- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy.Methods in molecular biology (Clifton, N.J.) · 2016Article
- TACC3 deregulates the DNA damage response and confers sensitivity to radiation and PARP inhibition.Oncogene · 2015Article
- The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase-independent manner.The Journal of biological chemistry · 2014Article
- Ste20-like protein kinase SLK (LOSK) regulates microtubule organization by targeting dynactin to the centrosome.Molecular biology of the cell · 2013Article
- TACC3 is essential for EGF-mediated EMT in cervical cancer.PloS one · 2013Article
- Augmin promotes meiotic spindle formation and bipolarity in Xenopus egg extracts.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.The Journal of cell biology · 2011Article
- Pulling it together: The mitotic function of TACC3.Bioarchitecture · 2011Article
- A critical role of integrin-linked kinase, ch-TOG and TACC3 in centrosome clustering in cancer cells.Oncogene · 2011Article
- Analysis of Dictyostelium TACC reveals differential interactions with CP224 and unusual dynamics of Dictyostelium microtubules.Cellular and molecular life sciences : CMLS · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing proteins of the tumor overexpressed gene (TOG) family to the centrosome and to facilitate their loading onto newly emerging microtubules. Using Xenopus egg extracts and in vitro assays, we show that the Xenopus TACC protein maskin is required for centrosome function beyond recruiting the Xenopus TOG protein XMAP215. The conserved C-terminal TACC domain of maskin is both necessary and sufficient to restore centrosome function in maskin-depleted extracts, and we provide evidence that the N terminus of maskin inhibits the function of the TACC domain. Time-lapse video microscopy reveals that microtubule dynamics in Xenopus egg extracts are unaffected by maskin depletion. Our results provide direct experimental evidence of a role for maskin in centrosome function and suggest that maskin is required for microtubule anchoring at the centrosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.