ArticleMolecular biology of the cell2008
Differences in regulation of Drosophila and vertebrate integrin affinity by talin.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Integrin αBiophysical journal · 2023Article
- Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.Experimental cell research · 2016Review
- Regulation of integrin-mediated adhesions.Current opinion in cell biology · 2015Review
- The talin head domain reinforces integrin-mediated adhesion by promoting adhesion complex stability and clustering.PLoS genetics · 2014Article
- Mechanisms of talin-dependent integrin signaling and crosstalk.Biochimica et biophysica acta · 2014Review
- Talins and kindlins: partners in integrin-mediated adhesion.Nature reviews. Molecular cell biology · 2013Review
- Defective retinal vascular endothelial cell development as a consequence of impaired integrin αVβ8-mediated activation of transforming growth factor-β.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Identification of integrin beta subunit mutations that alter affinity for extracellular matrix ligand.The Journal of biological chemistry · 2011Article
- Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.Molecular and cellular biology · 2011Article
- Integrin alphaIIbbeta3 activation in Chinese hamster ovary cells and platelets increases clustering rather than affinity.The Journal of biological chemistry · 2010Article
- The mechanical integrin cycle.Journal of cell science · 2009Review
- Integrin signalling at a glance.Journal of cell science · 2009Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Integrin-mediated cell adhesion is essential for development of multicellular organisms. In worms, flies, and vertebrates, talin forms a physical link between integrin cytoplasmic domains and the actin cytoskeleton. Loss of either integrins or talin leads to similar phenotypes. In vertebrates, talin is also a key regulator of integrin affinity. We used a ligand-mimetic Fab fragment, TWOW-1, to assess talin's role in regulating Drosophila alphaPS2 betaPS affinity. Depletion of cellular metabolic energy reduced TWOW-1 binding, suggesting alphaPS2 betaPS affinity is an active process as it is for vertebrate integrins. In contrast to vertebrate integrins, neither talin knockdown by RNA interference nor talin head overexpression had a significant effect on TWOW-1 binding. Furthermore, replacement of the transmembrane or talin-binding cytoplasmic domains of alphaPS2 betaPS with those of human alphaIIb beta3 failed to enable talin regulation of TWOW-1 binding. However, substitution of the extracellular and transmembrane domains of alphaPS2 betaPS with those of alphaIIb beta3 resulted in a constitutively active integrin whose affinity was reduced by talin knockdown. Furthermore, wild-type alphaIIb beta3 was activated by overexpression of Drosophila talin head domain. Thus, despite evolutionary conservation of talin's integrin/cytoskeleton linkage function, talin is not sufficient to regulate Drosophila alphaPS2 betaPS affinity because of structural features inherent in the alphaPS2 betaPS extracellular and/or transmembrane domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.