ArticleJournal of virology2008
Atomic force microscopy investigation of vaccinia virus structure.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Architecture and Assembly of Structurally Complex Viruses.Sub-cellular biochemistry · 2024Review
- Assembly and Evolution of Poxviruses.Advances in experimental medicine and biology · 2024Review
- Single-particle virology.Biophysical reviews · 2020Review
- The Vaccinia virion: Filling the gap between atomic and ultrastructure.PLoS pathogens · 2019Article
- Role of Condensing Particles in Polymer Confinement: A Model for Virus-Packed "Minichromosomes".Biophysical journal · 2017Article
- Review
- Nanostructured self-assembly of inverted formin 2 (INF2) and F-actin-INF2 complexes revealed by atomic force microscopy.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Development of a bio-analytical strategy for characterization of vaccine particles combining SEC and nanoES GEMMA.The Analyst · 2014Article
- Morphogenesis of mimivirus and its viral factories: an atomic force microscopy study of infected cells.Journal of virology · 2013Article
- Review
- Structure and assembly of complex viruses.Sub-cellular biochemistry · 2013Review
- Structural organization of DNA in chlorella viruses.PloS one · 2012Article
- Atomic force microscopy in imaging of viruses and virus-infected cells.Microbiology and molecular biology reviews : MMBR · 2011Review
- Investigation of bacteriophage T4 by atomic force microscopy.Bacteriophage · 2011Article
- Structural studies of the giant mimivirus.PLoS biology · 2009Article
- Monkeypox: A current emergency global health threat.Turkish journal of emergency medicineReview
- Diversity and evolution of chromatin proteins encoded by DNA viruses.Biochimica et biophysica actaReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vaccinia virus was treated in a controlled manner with various combinations of nonionic detergents, reducing agents, and proteolytic enzymes, and successive products of the reactions were visualized using atomic force microscopy (AFM). Following removal of the outer lipid/protein membrane, a layer 20 to 40 nm in thickness was encountered that was composed of fibrous elements which, under reducing conditions, rapidly decomposed into individual monomers on the substrate. Beneath this layer was the virus core and its prominent lateral bodies, which could be dissociated or degraded with proteases. The core, in addition to the lateral bodies, was composed of a thick, multilayered shell of proteins of diverse sizes and shapes. The shell, which was readily etched with proteases, was thoroughly permeated with pores, or channels. Prolonged exposure to proteases and reductants produced disgorgement of the viral DNA from the remainders of the cores and also left residual, flattened, protease-resistant sacs on the imaging substrate. The DNA was readily visualized by AFM, which revealed some regions to be "soldered" by proteins, others to be heavily complexed with protein, and yet other parts to apparently exist as bundled, naked DNA. Prolonged exposure to proteases deproteinized the DNA, leaving masses of extended, free DNA. Estimates of the interior core volume suggest moderate but not extreme compaction of the genome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.