ArticleJournal of virology2008
Asparagine 631 variants of the chicken Mx protein do not inhibit influenza virus replication in primary chicken embryo fibroblasts or in vitro surrogate assays.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Genetic resilience or resistance in poultry against avian influenza virus: mirage or reality?Journal of virology · 2025Review
- Effect of pandemic influenza A virus PB1 genes of avian origin on viral RNA polymerase activity and pathogenicity.Science advances · 2024Article
- Antiviral Effects of Avian Interferon-Stimulated Genes.Animals : an open access journal from MDPI · 2024Review
- Induction and antiviral activity of ferret myxovirus resistance (Mx) protein 1 against influenza A viruses.Scientific reports · 2024Article
- Anti-influenza A virus restriction factors that shape the human species barrier and virus evolution.PLoS pathogens · 2023Article
- Therapeutic Promises of Plant Metabolites against Monkeypox Virus: An In Silico Study.Advances in virology · 2023Article
- Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles.Nature microbiology · 2022Article
- Article
- Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells.Veterinarni medicina · 2021Article
- Review
- Dynamic Changes in the Expression of Interferon-Stimulated Genes in Joints of SPF Chickens Infected With Avian Reovirus.Frontiers in veterinary science · 2021Article
- Influenza A Virus-Host Specificity: An Ongoing Cross-Talk Between Viral and Host Factors.Frontiers in microbiology · 2021Review
- Pathobiology of the highly pathogenic avian influenza viruses H7N1 and H5N8 in different chicken breeds and role of Mx 2032 G/A polymorphism in infection outcome.Veterinary research · 2020Article
- Mx genes: host determinants controlling influenza virus infection and trans-species transmission.Human genetics · 2020Review
- Birth and death of Mx genes and the presence/absence of genes regulating Mx transcription are correlated with the diversity of anti-pathogenicity in vertebrate species.Molecular genetics and genomics : MGG · 2019Article
- Innate Immune Responses to Avian Influenza Viruses in Ducks and Chickens.Veterinary sciences · 2019Review
- Host and viral determinants of influenza A virus species specificity.Nature reviews. Microbiology · 2019Review
- Review
- Identification ofJournal, genetic engineering & biotechnology · 2018Article
- Transcriptional Innate Immune Response of the Developing Chicken Embryo to Newcastle Disease Virus Infection.Frontiers in genetics · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Whether chicken Mx inhibits influenza virus replication is an important question with regard to strategies aimed at enhancing influenza resistance in domestic flocks. The Asn631 polymorphism of the chicken Mx protein found in the Shamo (SHK) chicken line was previously reported to be crucial for the antiviral activity of this highly polymorphic chicken gene. Our aims were to determine whether cells from commercial chicken lines containing Asn631 alleles were resistant to influenza virus infection and to investigate the effects that other polymorphisms might have on Mx function. Unexpectedly, we found that the Asn631 genotype had no impact on multicycle replication of influenza virus (A/WSN/33 [H1N1]) in primary chicken embryo fibroblast lines. Furthermore, expression of the Shamo (SHK) chicken Mx protein in transfected 293T cells did not inhibit viral gene expression (A/PR/8/34 [H1N1], A/Duck/England/62 [H4N6], and A/Duck/Singapore/97 [H5N3]). Lastly, in minireplicon systems (A/PR/8/34 and A/Turkey/England/50-92/91 [H5N1]), which were highly sensitive to inhibition by the murine Mx1 and human MxA proteins, respectively, Shamo chicken Mx also proved ineffective in the context of avian as well as mammalian cell backgrounds. Our findings demonstrate that Asn631 chicken Mx alleles do not inhibit influenza virus replication of the five strains tested here and efforts to increase the frequency of Asn631 alleles in commercial chicken populations are not warranted. Nevertheless, chicken Mx variants with anti-influenza activity might still exist. The flow cytometry and minireplicon assays described herein could be used as efficient functional screens to identify such active chicken Mx alleles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.