ArticleJournal of molecular biology2008
Cross-linking of phospholipid membranes is a conserved property of calcium-sensitive synaptotagmins.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms.Biophysical journal · 2020Article
- Doc2b CaScientific reports · 2019Article
- PI(4,5)P₂-binding effector proteins for vesicle exocytosis.Biochimica et biophysica acta · 2015Review
- Control of membrane gaps by synaptotagmin-Ca2+ measured with a novel membrane distance ruler.Nature communications · 2014Article
- Prevalent mechanism of membrane bridging by synaptotagmin-1.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment.Nature structural & molecular biology · 2013Article
- Solution single-vesicle assay reveals PIP2-mediated sequential actions of synaptotagmin-1 on SNAREs.The EMBO journal · 2012Article
- Synaptotagmin 1 modulates lipid acyl chain order in lipid bilayers by demixing phosphatidylserine.The Journal of biological chemistry · 2011Article
- Forming giant vesicles with controlled membrane composition, asymmetry, and contents.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Mechanism and function of synaptotagmin-mediated membrane apposition.Nature structural & molecular biology · 2011Article
- Synaptic vesicles studied by dynamic light scattering.The European physical journal. E, Soft matter · 2011Article
- Phosphatidylinositol 4,5-bisphosphate alters synaptotagmin 1 membrane docking and drives opposing bilayers closer together.Biochemistry · 2011Article
- Synaptotagmin 1 and SNAREs form a complex that is structurally heterogeneous.Journal of molecular biology · 2011Article
- HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly.Molecular biology of the cell · 2010Article
- Minimal membrane docking requirements revealed by reconstitution of Rab GTPase-dependent membrane fusion from purified components.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Solution and membrane-bound conformations of the tandem C2A and C2B domains of synaptotagmin 1: Evidence for bilayer bridging.Journal of molecular biology · 2009Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Synaptotagmins are vesicular proteins implicated in many membrane trafficking events. They are highly conserved in evolution and the mammalian family contains 16 isoforms. We now show that the tandem C2 domains of several calcium-sensitive synaptotagmin isoforms tested, including Drosophila synaptotagmin, rapidly cross-link phospholipid membranes. In contrast to the tandem structure, individual C2 domains failed to trigger membrane cross-linking in several novel assays. Large-scale liposomal aggregation driven by tandem C2 domains in response to calcium was confirmed by the following techniques: turbidity assay, dynamic light-scattering and both confocal and negative stain electron microscopy. Firm cross-linking of membranes was evident from laser trap experiments. High-resolution cryo-electron microscopy revealed that membrane cross-linking by tandem C2 domains results in a constant distance of approximately 9 nm between the apposed membranes. Our findings show the conserved nature of this important property of synaptotagmin, demonstrate the significance of the tandem C2 domain structure and provide a plausible explanation for the accelerating effect of synaptotagmins on membrane fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.