ArticleThe EMBO journal1991
Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Profile of Helen M. Piwnica-Worms.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Tyrosine phosphoproteome profiling identifies cell-intrinsic signals limiting the efficacy of tyrosine kinase inhibitor therapies.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Medulloblastoma epigenetics and the path to clinical innovation.Journal of neuro-oncology · 2020Review
- Post-translational modifications at the ATP-positioning G-loop that regulate protein kinase activity.Pharmacological research · 2018Review
- Secretion modification region-derived peptide blocks exosome release and mediates cell cycle arrest in breast cancer cells.Oncotarget · 2017Article
- Glucose restriction induces transient G2 cell cycle arrest extending cellular chronological lifespan.Scientific reports · 2016Article
- Neuronal cell cycle: the neuron itself and its circumstances.Cell cycle (Georgetown, Tex.) · 2015Review
- Identification of a Wee1-like kinase gene essential for procyclic Trypanosoma brucei survival.PloS one · 2013Article
- Hyphal differentiation induced via a DNA damage checkpoint-dependent pathway engaged in crosstalk with nutrient stress signaling in Schizosaccharomyces japonicus.Current genetics · 2012Article
- Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Short-term integration of Cdc25 dynamics controls mitotic entry during Drosophila gastrulation.Developmental cell · 2012Article
- Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.Molecular biology of the cell · 2011Article
- DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.PloS one · 2011Article
- Transformation/transcription domain-associated protein (TRRAP)-mediated regulation of Wee1.Genetics · 2010Article
- Article
- Multisite M-phase phosphorylation of Xenopus Wee1A.Molecular and cellular biology · 2005Article
- Normal cell cycle and checkpoint responses in mice and cells lacking Cdc25B and Cdc25C protein phosphatases.Molecular and cellular biology · 2005Article
- Aurora kinase inhibitor ZM447439 blocks chromosome-induced spindle assembly, the completion of chromosome condensation, and the establishment of the spindle integrity checkpoint in Xenopus egg extracts.Molecular biology of the cell · 2005Article
- Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding.Molecular and cellular biology · 2003Article
- Inhibition of Chk1-dependent G2 DNA damage checkpoint radiosensitizes p53 mutant human cells.Oncogene · 2001Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
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Abstract
The regulation of p34cdc2 was investigated by overproducing p34cdc2, cyclin (A and B) and the wee1+ gene product (p107wee1) using a baculoviral expression system. p34cdc2 formed a functional complex with both cyclins as judged by co-precipitation, phosphorylation of cyclin in vitro, and activation of p34cdc2 histone H1 kinase activity. Co-production of p34cdc2 and p107wee1 in insect cells resulted in a minor population of p34cdc2 that was phosphorylated on tyrosine and displayed an altered electrophoretic mobility. When p34cdc2 and p107wee1 were co-produced with cyclin (A or B) in insect cells, there was a dramatic increase in the population of p34cdc2 that was phosphorylated on tyrosine and that displayed a shift in electrophoretic mobility. The phosphorylation of p34cdc2 on tyrosine was absolutely dependent upon the presence of kinase-active p107wee1. Tyrosine-specific as well as serine/threonine-specific protein kinase activities co-immunoprecipitated with p107wee1. These results suggest that cyclin functions to facilitate tyrosine phosphorylation of p34cdc2 and that p107wee1 functions to regulate p34cdc2, either directly or indirectly, by tyrosine phosphorylation.
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