Evidence map›Paper›PMID 18505806›Full record

ArticleInfection and immunity2008

Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts.

Philip A Arlen, Michael Singleton, Jeffrey J Adamovicz, Yi Ding, Abdolreza Davoodi-Semiromi, Henry Daniell

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
11.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 128 citations in OpenAlex.

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  13. Plant Platforms for Efficient Heterologous Protein Production.Biotechnology and bioprocess engineering : BBE · 2021
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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Philip A ArlenDepartment of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32816-2364, USA.
Michael Singleton
Jeffrey J Adamovicz
Yi Ding
Abdolreza Davoodi-Semiromi
Henry Daniell
University of Central Florida · USUnited States Army Medical Research Institute of Infectious Diseases · US

Funding

Expression of Human Therapeutic Proteins in Transgenic Tobacco ChloroplastsR01GM063879 · NIGMS · UNIVERSITY OF CENTRAL FLORIDA · PI DANIELL, HENRY · 2002 to 2010
$2.0M
NIGMS NIH HHS 5R01 GM 63879-06NIGMS NIH HHS R01 GM063879
6 · The paper itself

Abstract

The chloroplast bioreactor is an alternative to fermentation-based systems for production of vaccine antigens and biopharmaceuticals. We report here expression of the plague F1-V fusion antigen in chloroplasts. Site-specific transgene integration and homoplasmy were confirmed by PCR and Southern blotting. Mature leaves showed the highest level of transgene expression on the third day of continuous illumination, with a maximum level of 14.8% of the total soluble protein. Swiss Webster mice were primed with adjuvant-containing subcutaneous (s.c.) doses of F1-V and then boosted with either adjuvanted s.c. doses (s.c. F1-V mice) or unadjuvanted oral doses (oral F1-V mice). Oral F1-V mice had higher prechallenge serum immunoglobulin G1 (IgG1) titers than s.c. F1-V mice. The corresponding serum levels of antigen-specific IgG2a and IgA were 2 and 3 orders of magnitude lower, respectively. After vaccination, mice were exposed to an inhaled dose of 1.02 x 10(6) CFU of aerosolized Yersinia pestis CO92 (50% lethal dose, 6.8 x 10(4) CFU). All control animals died within 3 days. F1-V given s.c. (with adjuvant) protected 33% of the immunized mice, while 88% of the oral F1-V mice survived aerosolized Y. pestis challenge. A comparison of splenic Y. pestis CFU counts showed that there was a 7- to 10-log reduction in the mean bacterial burden in survivors. Taken together, these data indicate that oral booster doses effectively elicit protective immune responses in vivo. In addition, this is the first report of a plant-derived oral vaccine that protected animals from live Y. pestis challenge, bringing the likelihood of lower-cost vaccines closer to reality.

Indexed as

Administration, OralAnimalsAntibodies, BacterialAntigens, BacterialBacterial ProteinsChloroplastsColony Count, MicrobialFemaleImmunization, SecondaryImmunoglobulin AImmunoglobulin GInjections, SubcutaneousMicePlaguePlague VaccinePlants, Genetically ModifiedAntibodies, BacterialAntigens, BacterialBacterial Proteinscaf1 protein, Yersinia pestisImmunoglobulin AImmunoglobulin GLcrV protein, YersiniaPlague VaccinePore Forming Cytotoxic ProteinsRecombinant Fusion Proteins

Identifiers

PMID18505806
PMCPMC2493203
OpenAlexW2026386446

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.