ArticleProtein science : a publication of the Protein Society2008
Crystal structure and mutagenic analysis of GDOsp, a gentisate 1,2-dioxygenase from Silicibacter pomeroyi.
Article in Protein science : a publication of the Protein Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 54 citations in OpenAlex.
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- Crystal structure of the monocupin ring-cleaving dioxygenase 5-nitrosalicylate 1,2-dioxygenase from Bradyrhizobium sp.Acta crystallographica. Section D, Structural biology · 2023Article
- Spectroscopic analysis of the mammalian enzyme cysteine dioxygenase.Methods in enzymology · 2023Article
- Differences in the Second Coordination Sphere Tailor the Substrate Specificity and Reactivity of Thiol Dioxygenases.Accounts of chemical research · 2022Article
- Spectroscopic Investigation of Cysteamine Dioxygenase.Biochemistry · 2020Article
- Substrate promiscuity and active site differences in gentisate 1,2-dioxygenases: electron paramagnetic resonance study.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2019Article
- A Biomimetic System for Studying Salicylate Dioxygenase.ACS symposium series. American Chemical Society · 2019Article
- A Structural Model for the Iron-Nitrosyl Adduct of Gentisate Dioxygenase.European journal of inorganic chemistry · 2018Article
- Synthesis, X-ray Structures, Electronic Properties, and OInorganic chemistry · 2016Article
- Identification and Characterization of a Novel Gentisate 1,2-Dioxygenase Gene from a Halophilic Martelella Strain.Scientific reports · 2015Article
- Nickel quercetinase, a "promiscuous" metalloenzyme: metal incorporation and metal ligand substitution studies.BMC biochemistry · 2015Article
- Spectroscopic and computational investigation of iron(III) cysteine dioxygenase: implications for the nature of the putative superoxo-Fe(III) intermediate.Biochemistry · 2014Article
- Structure of the 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Structure and function of atypically coordinated enzymatic mononuclear non-heme-Fe(II) centers.Coordination chemistry reviews · 2013Review
- Structural, spectroscopic, and electrochemical properties of nonheme Fe(II)-hydroquinonate complexes: synthetic models of hydroquinone dioxygenases.Dalton transactions (Cambridge, England : 2003) · 2012Article
- Crystallization and preliminary X-ray crystallographic analysis of hydroquinone dioxygenase from Sphingomonas sp. TTNP3.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Ring-cleaving dioxygenases with a cupin fold.Applied and environmental microbiology · 2012Review
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10 authors at 6 institutions in 2 countries.
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Abstract
Dioxygenases catalyze dioxygen incorporation into various organic compounds and play a key role in the complex degradation pathway of mono- and polycyclic aromatic and hetero-aromatic compounds. Here we report the crystal structure of gentisate 1,2-dioxygenase from Silicibacter pomeroyi (GDOsp) at a 2.8 A resolution. The enzyme possessed a conserved three-dimensional structure of the bicupin family, forming a homotetramerization. However, each subunit of GDOsp unusually contained two ferrous centers that were located in its two homologous cupin domains, respectively. Further mutagenic analysis indicated that the enzyme activity of GDOsp depends on the microenvironment in both metal-binding sites. Moreover, homologous structural comparison and functional study on GDOsp variants unveiled a group of functionally essential residues and suggested that the active site of the enzyme is located in the amino-terminal domain, but could be influenced by changes in the carboxyl domain. Therefore, GDOsp may provide a working model for studying long-distance communication within a protein (or its complex).
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