ArticleJournal of cellular and molecular medicine2010
Foetal hepatocyte transplantation in a vascularized AV-Loop transplantation model in the rat.
Article in Journal of cellular and molecular medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Indirect 3D Bioprinting of a Robust Trilobular Hepatic Construct with Decellularized Liver Matrix Hydrogel.Bioengineering (Basel, Switzerland) · 2022Article
- Aberrant Differentiation of Human Pluripotent Stem Cell-Derived Kidney Precursor Cells inside Mouse Vascularized Bioreactors.Nephron · 2020Article
- Angiogenesis and tissue formation driven by an arteriovenous loop in the mouse.Scientific reports · 2019Article
- Generation of Functioning Nephrons by Implanting Human Pluripotent Stem Cell-Derived Kidney Progenitors.Stem cell reports · 2018Article
- From human pluripotent stem cells to functional kidney organoids and models of renal disease.Stem cell investigation · 2018Article
- Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues.ACS biomaterials science & engineering · 2016Article
- The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering.Journal of visualized experiments : JoVE · 2016Article
- Vessel transformation in chronic wounds under topical negative pressure therapy: an immunohistochemical analysis.International wound journal · 2015Article
- Regenerative surgery: tissue engineering in general surgical practice.World journal of surgery · 2012Review
- Tissue engineering and regenerative medicine -where do we stand?Journal of cellular and molecular medicine · 2012Review
- Endothelial progenitor cells are integrated in newly formed capillaries and alter adjacent fibrovascular tissue after subcutaneous implantation in a fibrin matrix.Journal of cellular and molecular medicine · 2011Article
- Biomaterials to prevascularize engineered tissues.Journal of cardiovascular translational research · 2011Review
- The reconstruction of lung alveolus-like structure in collagen-matrigel/microcapsules scaffolds in vitro.Journal of cellular and molecular medicine · 2011Article
- Changes of anabolic processes at the cellular and molecular level in chronic wounds under topical negative pressure can be revealed by transcriptome analysis.Journal of cellular and molecular medicine · 2011Article
- Engineering skeletal muscle tissue--new perspectives in vitro and in vivo.Journal of cellular and molecular medicine · 2010Review
- Regenerative medicine: then and now--an update of recent history into future possibilities.Journal of cellular and molecular medicine · 2010Review
- [The development of plastic surgery: retrospective view of 80 years of "Der Chirurg" (The Surgeon)].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2009Article
- Translating tissue engineering technology platforms into cancer research.Journal of cellular and molecular medicine · 2009Review
- Development of hepatic tissue engineering.Pediatric surgery international · 2009Review
- Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscle.BMC biotechnology · 2009Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of foetal liver cells (FLC) in the context of hepatic tissue engineering might permit efficient in vitro expansion and cryopreservation in a cell bank. A prerequisite for successful application of bioartificial liver tissue is sufficient initial vascularization. In this study, we evaluated the transplantation of fibrin gel-immobilized FLC in a vascularized arterio-veno-venous (AV)-loop model. FLC were isolated from embryonic/foetal (ED 16) rat livers and were enriched by using magnetic cell sorting (MACS). After cryopreservation, FLC were labelled by pkh-26. Cells were transplanted in a fibrin matrix into a subcutaneous chamber containing a microsurgically created AV-loop in the femoral region of the recipient rat. The chambers were explanted after 14 days. Subcutaneous implants without an AV-loop and cell-free implants served as controls. Fluorescence microscopy of the constructs was used to identify pkh-26(+)- donor cells. Characterization was performed by RT-PCR and immunhistology (IH) for CK-18 and CD31. Transplantation of FLC using the AV-loop permitted a neo-tissue formation in the fibrin matrix. A high-density vascularization was observed in the AV-loop constructs as shown by CD31 IH. Viable foetal donor cells were detected which expressed CK-18. FLC can be successfully used for heterotopic transplantation. Fibrin matrix permits rapid blood vessel ingrowth from the AV-loop and supports engraftment of FLC. It is therefore an appropriate environment for hepatocyte transplantation in combination with microsurgical vascularization strategies. Transplantation of fibrin gel-immobilized FLC may be a promising approach for the development of highly vascularized in vivo tissue-engineering-based liver support systems.
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