ArticleThe Journal of general physiology2008
Surface expression of epithelial Na channel protein in rat kidney.
Article in The Journal of general physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 104 citations in OpenAlex.
- Albuminuria is associated with an increased prostasin in urine while aldosterone has no direct effect on urine and kidney tissue abundance of prostasin.Pflugers Archiv : European journal of physiology · 2017Trial
- ENaC processing in rat kidney: effects of salt loading and ADH.American journal of physiology. Renal physiology · 2026Article
- Processing of ENaC in mouse kidney: effects of aldosterone and a Liddle syndrome mutation.American journal of physiology. Renal physiology · 2026Article
- Kinetics of aldosterone-dependent ENaC trafficking in the kidney.The Journal of general physiology · 2025Article
- Transmembrane Serine Protease 2 and Proteolytic Activation of the Epithelial Sodium Channel in Mouse Kidney.Journal of the American Society of Nephrology : JASN · 2025Article
- Structural insights into subunit-dependent functional regulation in epithelial sodium channels.Structure (London, England : 1993) · 2025Article
- A Possible Link between Cell Plasticity and Renin Expression in the Collecting Duct: A Narrative Review.International journal of molecular sciences · 2024Review
- Control of ENaC ubiquitination.American journal of physiology. Renal physiology · 2024Article
- Effects of a high-salt diet on MAP and expression levels of renal ENaCs and aquaporins in SHR.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- Dietary anions control potassium excretion: it is more than a poorly absorbable anion effect.American journal of physiology. Renal physiology · 2023Article
- High dietary KAmerican journal of physiology. Renal physiology · 2023Article
- Aldosterone: Renal Action and Physiological Effects.Comprehensive Physiology · 2023Article
- Article
- Expression of ENaC subunits in epithelia.The Journal of general physiology · 2022Article
- Does the early aldosterone-induced SGK1 play a role in early Kaliuresis?Physiological reports · 2022Article
- Cleavage state of γENaC in mouse and rat kidneys.American journal of physiology. Renal physiology · 2021Article
- ENaC expression correlates with the acute furosemide-induced KPhysiological reports · 2021Article
- Aldosterone-dependent and -independent regulation of NaAmerican journal of physiology. Renal physiology · 2020Article
- Ubiquitination of renal ENaC subunits in vivo.American journal of physiology. Renal physiology · 2020Article
- The renal and blood pressure response to low sodium diet in P2X4 receptor knockout mice.Physiological reports · 2018Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Expression of epithelial Na channel (ENaC) protein in the apical membrane of rat kidney tubules was assessed by biotinylation of the extracellular surfaces of renal cells and by membrane fractionation. Rat kidneys were perfused in situ with solutions containing NHS-biotin, a cell-impermeant biotin derivative that attaches covalently to free amino groups on lysines. Membranes were solubilized and labeled proteins were isolated using neutravidin beads, and surface beta and gammaENaC subunits were assayed by immunoblot. Surface alphaENaC was assessed by membrane fractionation. Most of the gammaENaC at the surface was smaller in molecular mass than the full-length subunit, consistent with cleavage of this subunit in the extracellular moiety close to the first transmembrane domains. Insensitivity of the channels to trypsin, measured in principal cells of the cortical collecting duct by whole-cell patch-clamp recording, corroborated this finding. ENaC subunits could be detected at the surface under all physiological conditions. However increasing the levels of aldosterone in the animals by feeding a low-Na diet or infusing them directly with hormone via osmotic minipumps for 1 wk before surface labeling increased the expression of the subunits at the surface by two- to fivefold. Salt repletion of Na-deprived animals for 5 h decreased surface expression. Changes in the surface density of ENaC subunits contribute significantly to the regulation of Na transport in renal cells by mineralocorticoid hormone, but do not fully account for increased channel activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.