ArticleApplied and environmental microbiology2008
Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed.
- New Methodologies as Opportunities in the Study of Bacterial Biofilms, Including Food-Related Applications.Microorganisms · 2025Review
- Integrating Bacteriocins and Biofilm-Degrading Enzymes to EliminateInternational journal of molecular sciences · 2025Article
- A short-lived peptide signal regulates cell-to-cell communication in Listeria monocytogenes.Communications biology · 2024Article
- Characterization of an Autoinducing Peptide Signal Reveals Highly Efficacious Synthetic Inhibitors and Activators of Quorum Sensing and Biofilm Formation inBiochemistry · 2023Article
- Review
- The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells.NPJ biofilms and microbiomes · 2023Article
- Article
- TheFrontiers in microbiology · 2023Article
- The role of the Listeria monocytogenes surfactome in biofilm formation.Microbial biotechnology · 2021Review
- Characterization of the biofilm phenotype of a Listeria monocytogenes mutant deficient in agr peptide sensing.MicrobiologyOpen · 2019Article
- Biofilm Formation ofFrontiers in microbiology · 2019Article
- Microbial community dynamics during aerobic granulation in a sequencing batch reactor (SBR).PeerJ · 2019Article
- Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to Biocides.Applied and environmental microbiology · 2018Article
- MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence.Nucleic acids research · 2018Article
- Article
- VLP-based vaccine induces immune control of Staphylococcus aureus virulence regulation.Scientific reports · 2017Article
- Modeling Reveals the Role of Aging and Glucose Uptake Impairment in L1A1Frontiers in microbiology · 2017Article
- Impact of Nutrient Restriction on the Structure ofFrontiers in microbiology · 2017Article
- Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria.Applied and environmental microbiology · 2016Review
- A Look inside the Listeria monocytogenes Biofilms Extracellular Matrix.Microorganisms · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in the food industry. Biofilms are three-dimensional microbial structures that facilitate the persistence of pathogens on surfaces, their resistance toward antimicrobials, and the final contamination of processed goods. So far, little is known about the structural dynamics of L. monocytogenes biofilm formation and its regulation. The aims of this study were, by combining genetics and time-lapse laser-scanning confocal microscopy (LSCM), (i) to characterize the structural dynamics of L. monocytogenes EGD-e sessile growth in two nutritional environments (with or without a nutrient flow), and (ii) to evaluate the possible role of the L. monocytogenes agr system during biofilm formation by tracking the spatiotemporal fluorescence expression of a green fluorescent protein (GFP) reporter system. In the absence of nutrient flow (static conditions), unstructured biofilms composed of a few layers of cells that covered the substratum were observed. In contrast, when grown under dynamic conditions, L. monocytogenes EGD-e biofilms were highly organized. Indeed, ball-shaped microcolonies were surrounded by a network of knitted chains. The spatiotemporal tracking of fluorescence emitted by the GFP reporter system revealed that agr expression was barely detectable under static conditions, but it progressively increased during 40 h under dynamic conditions. Moreover, spatial analysis revealed that agr was expressed preferentially in cells located outside the microcolonies. Finally, the in-frame deletion of agrA, which encodes a transcriptional regulator, resulted in a decrease in initial adherence without affecting the subsequent biofilm development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.