ArticleJournal of bacteriology2008
Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 174 citations in OpenAlex.
- Uncovering potential autotrophs in coal seams: characterization, enrichment, and genome-resolved metabolisms of a subsurface coal seam microbiome.Applied and environmental microbiology · 2026Article
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- FormatotrophicSynthetic and systems biotechnology · 2026Article
- High-efficiency methane consumption by atmospheric methanotrophs in subsurface karst caves: The irrefutable methane sink.Science advances · 2026Article
- An Efficient COAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Methanotrophs and methane oxidation in cold wetlands: distribution, ecology, and mechanisms.ISME communications · 2026Review
- XoxF and the Calvin-Benson cycle mediate lanthanide-dependent growth on methanol inApplied and environmental microbiology · 2025Article
- Diel cycle of lanthanide-dependent methylotrophy by TMED127/Methylaequorales bacteria in oligotrophic surface seawater.Applied and environmental microbiology · 2025Article
- EngineeredmSphere · 2025Article
- Non-growth substrate ethane perturbs core methanotrophy in obligate methanotrophApplied and environmental microbiology · 2025Article
- Methylobacterium extorquens PA1 utilizes multiple strategies to maintain formaldehyde homeostasis during methylotrophic growth.PLoS genetics · 2025Article
- Advancing yeast metabolism for a sustainable single carbon bioeconomy.FEMS yeast research · 2025Review
- Modification and analysis of context-specific genome-scale metabolic models: methane-utilizing microbial chassis as a case study.mSystems · 2025Article
- A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates.bioRxiv : the preprint server for biology · 2024Article
- Single carbon metabolism - A new paradigm for microbial bioprocesses?Synthetic and systems biotechnology · 2024Review
- Physiological basis for atmospheric methane oxidation and methanotrophic growth on air.Nature communications · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CONature communications · 2023Article
- Tailored extraction and ion mobility-mass spectrometry enables isotopologue analysis of tetrahydrofolate vitamers.Analytical and bioanalytical chemistry · 2023Article
- Metabolic engineering strategies for microbial utilization of methanol.Engineering microbiology · 2023Review
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In serine cycle methylotrophs, methylene tetrahydrofolate (H4F) is the entry point of reduced one-carbon compounds into the serine cycle for carbon assimilation during methylotrophic metabolism. In these bacteria, two routes are possible for generating methylene H4F from formaldehyde during methylotrophic growth: one involving the reaction of formaldehyde with H4F to generate methylene H4F and the other involving conversion of formaldehyde to formate via methylene tetrahydromethanopterin-dependent enzymes and conversion of formate to methylene H4F via H4F-dependent enzymes. Evidence has suggested that the direct condensation reaction is the main source of methylene H4F during methylotrophic metabolism. However, mutants lacking enzymes that interconvert methylene H4F and formate are unable to grow on methanol, suggesting that this route for methylene H4F synthesis should have a significant role in biomass production during methylotrophic metabolism. This problem was investigated in Methylobacterium extorquens AM1. Evidence was obtained suggesting that the existing deuterium assay might overestimate the flux through the direct condensation reaction. To test this possibility, it was shown that only minor assimilation into biomass occurred in mutants lacking the methylene H4F synthesis pathway through formate. These results suggested that the methylene H4F synthesis pathway through formate dominates assimilatory flux. A revised kinetic model was used to validate this possibility, showing that physiologically plausible parameters in this model can account for the metabolic fluxes observed in vivo. These results all support the suggestion that formate, not formaldehyde, is the main branch point for methylotrophic metabolism in M. extorquens AM1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.