ArticleBiophysical journal2008
Giant unilamellar vesicles containing phosphatidylinositol(4,5)bisphosphate: characterization and functionality.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 106 citations in OpenAlex.
- Selective membrane wetting of phase-separated giant unilamellar vesicles by coacervate droplets.Nature communications · 2026Article
- Combined thermodynamic and time-resolved structural analysis of interactions between AP2 and biomimetic plasma membranes provides insights into clathrin-mediated endocytosis.Communications biology · 2025Article
- Single-Vesicle Microelectroanalysis Reveals the Role of PIP2 Phospholipid in Vesicle Opening Dynamics and Its Potential Role in Exocytosis.ACS omega · 2025Article
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- Unlocking the power of membrane biophysics: enhancing the study of antimicrobial peptides activity and selectivity.Biophysical reviews · 2025Review
- Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.Biophysical journal · 2024Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Reconstitution of ATP-dependent lipid transporters: gaining insight into molecular characteristics, regulation, and mechanisms.Bioscience reports · 2023Review
- Temperature-Promoted Giant Unilamellar Vesicle (GUV) Aggregation: A Way of Multicellular Formation.Current issues in molecular biology · 2023Article
- Scratching beyond the surface - minimal actin assemblies as tools to elucidate mechanical reinforcement and shape change.Emerging topics in life sciences · 2022Article
- Review
- Insights into Membrane Curvature Sensing and Membrane Remodeling by Intrinsically Disordered Proteins and Protein Regions.The Journal of membrane biology · 2022Review
- A biophysical perspective of the regulatory mechanisms of ezrin/radixin/moesin proteins.Biophysical reviews · 2022Review
- Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results.Membranes · 2021Review
- PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles.Bioinformatics advances · 2021Article
- Article
- On-Chip Inverted Emulsion Method for Fast Giant Vesicle Production, Handling, and Analysis.Micromachines · 2020Article
- PIP2 Reshapes Membranes through Asymmetric Desorption.Biophysical journal · 2019Article
- Tunable Membrane Potential Reconstituted in Giant Vesicles Promotes Permeation of Cationic Peptides at Nanomolar Concentrations.ACS applied materials & interfaces · 2018Article
- A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programing.Nature communications · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomimetic systems such as giant unilamellar vesicles (GUVs) are increasingly used for studying protein/lipid interactions due to their size (similar to that of cells) and to their ease of observation by light microscopy techniques. Biophysicists have begun to complexify GUVs to investigate lipid/protein interactions. In particular, composite GUVs have been designed that incorporate lipids that play important physiological roles in cellulo, such as phosphoinositides and among those the most abundant one, phosphatidylinositol(4,5)bisphosphate (PIP2). Fluorescent lipids are often used as tracers to observe GUV membranes by microscopy but they can not bring quantitative information about the insertion of unlabeled lipids. In this study, we carried out zeta-potential measurements to prove the effective incorporation of PIP2 as well as that of phosphatidylserine in the membrane of GUVs prepared by electroformation and to follow the stability of PIP2-containing GUVs. Using confocal microscopy, we found that long-chain (C16) fluorescent PIP2 analogs used as tracers (0.1% of total lipids) show a uniform distribution in the membrane whereas PIP2 antibodies show PIP2 clustering. However, the clustering effect, which is emphasized when tertiary antibodies are used in addition to secondary ones to enhance the size of the detection complex, is artifactual. We showed that divalent ions (Ca2+ and Mg2+) can induce aggregation of PIP2 in the membrane depending on their concentration. Finally, the interaction of ezrin with PIP2-containing GUVs was investigated. Using either labeled ezrin and unlabeled GUVs or both labeled ezrin and GUVs, we showed that clusters of PIP2 and proteins are formed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.