ArticleBiophysical journal2008
Localized acetylcholine receptor clustering dynamics in response to microfluidic focal stimulation with agrin.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Neuromuscular Development and Disease: Learning FromFrontiers in cell and developmental biology · 2021Review
- Engineering 3D skeletal muscle primed for neuromuscular regeneration following volumetric muscle loss.Biomaterials · 2020Article
- Vascularized and Innervated Skeletal Muscle Tissue Engineering.Advanced healthcare materials · 2020Review
- Toward Building the Neuromuscular Junction: In Vitro Models To Study Synaptogenesis and Neurodegeneration.ACS omega · 2019Review
- Challenges and Future Prospects on 3DFrontiers in bioengineering and biotechnology · 2018Review
- An open-chamber flow-focusing device for focal stimulation of micropatterned cells.Biomicrofluidics · 2016Article
- Biomimetic tissues on a chip for drug discovery.Drug discovery today · 2012Review
- Soluble miniagrin enhances contractile function of engineered skeletal muscle.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Design and dynamic characterization of "single-stroke" peristaltic PDMS micropumps.Lab on a chip · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Agrin is a proteoglycan secreted by the motor neuron's growing axon terminal upon contact with the muscle during embryonic development. It was long thought that agrin's role was to trigger the clustering of acetylcholine receptors (AChRs) to nascent synapse sites. However, agrin-predating, protosynaptic AChR clusters are present well before innervation in the embryo and in myotube cultures, yet no role has been conclusively ascribed to agrin. We used a microfluidic device to focally deliver agrin to protosynaptic AChR clusters in micropatterned myotube cultures. The distribution of AChRs labeled with fluorescent bungarotoxin was imaged at various time points over >24 h. We find that a 4-h focal application of agrin (100 nM) preferentially reduces AChR loss at agrin-exposed clusters by 17% relative to the agrin-deprived clusters on the same myotube. In addition, the focal application increases the addition of AChRs preferentially at the clusters by 10% relative to the agrin-exposed, noncluster areas. Taken together, these findings suggest that a focal agrin stimulus can play a key stabilizing role in the aggregation of AChRs at the early stages of synapse formation. This methodology is generally applicable to various developmental processes and cell types, including neurons and stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.