ArticleThe Journal of biological chemistry2008
Structure and mechanism of the rebeccamycin sugar 4'-O-methyltransferase RebM.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
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- Structural Basis of Polyketide Synthase O-Methylation.ACS chemical biology · 2018Article
- The Structure of the Bifunctional Everninomicin Biosynthetic Enzyme EvdMO1 Suggests Independent Activity of the Fused Methyltransferase-Oxidase Domains.Biochemistry · 2018Article
- Biosynthetic studies on teleocidins in Streptomyces.The Journal of antibiotics · 2018Review
- Structural and functional characterization of the Vindoline biosynthesis pathway enzymes of Catharanthus roseus.Journal of molecular modeling · 2018Article
- Structural insights into the methyl donor recognition model of a novel membrane-binding protein UbiG.Scientific reports · 2016Article
- The Phormidolide Biosynthetic Gene Cluster: A trans-AT PKS Pathway Encoding a Toxic Macrocyclic Polyketide.Chembiochem : a European journal of chemical biology · 2016Article
- Cytotoxic Indolocarbazoles from Actinomadura melliaura ATCC 39691.Journal of natural products · 2015Article
- Structure and Biophysical Characterization of the S-Adenosylmethionine-dependent O-Methyltransferase PaMTH1, a Putative Enzyme Accumulating during Senescence of Podospora anserina.The Journal of biological chemistry · 2015Article
- Structural basis of substrate specificity and regiochemistry in the MycF/TylF family of sugar O-methyltransferases.ACS chemical biology · 2015Article
- Characterization of the calicheamicin orsellinate C2-O-methyltransferase CalO6.Chembiochem : a European journal of chemical biology · 2014Article
- Facile chemoenzymatic strategies for the synthesis and utilization of S-adenosyl-(L)-methionine analogues.Angewandte Chemie (International ed. in English) · 2014Article
- Structural and functional characterization of CalS11, a TDP-rhamnose 3'-O-methyltransferase involved in calicheamicin biosynthesis.ACS chemical biology · 2013Article
- Genetic insights into pyralomicin biosynthesis in Nonomuraea spiralis IMC A-0156.Journal of natural products · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The 2.65-angstroms crystal structure of the rebeccamycin 4'-O-methyltransferase RebM in complex with S-adenosyl-l-homocysteine revealed RebM to adopt a typical S-adenosylmethionine-binding fold of small molecule O-methyltransferases (O-MTases) and display a weak dimerization domain unique to MTases. Using this structure as a basis, the RebM substrate binding model implicated a predominance of nonspecific hydrophobic interactions consistent with the reported ability of RebM to methylate a wide range of indolocarbazole surrogates. This model also illuminated the three putative RebM catalytic residues (His140/141 and Asp166) subsequently found to be highly conserved among sequence-related natural product O-MTases from GC-rich bacteria. Interrogation of these residues via site-directed mutagenesis in RebM demonstrated His140 and Asp166 to be most important for catalysis. This study reveals RebM to be a member of the general acid/base-dependent O-MTases and, as the first crystal structure for a sugar O-MTase, may also present a template toward the future engineering of natural product MTases for combinatorial applications.
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