ArticleThe Journal of biological chemistry2008
Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of dolichols in plants.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 84 citations in OpenAlex.
- T2T and chromosome-level genome assemblies provide insights into the genetic basis of bioactive compound biosynthesis and environmental adaptation in licorice.Science China. Life sciences · 2026Article
- Farnesol and geranylgeraniol in plant reproduction: insights from Arabidopsis and beyond.Journal of experimental botany · 2026Review
- TPS-mediated transcription reprogramming during the robust aroma formation in Daphne odora flowers.BMC plant biology · 2026Article
- Advances in the Biosynthesis of Plant Terpenoids: Models, Mechanisms, and Applications.Plants (Basel, Switzerland) · 2025Review
- HDR, the last enzyme in the MEP pathway, differently regulates isoprenoid biosynthesis in two woody plants.Plant physiology · 2023Article
- Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis.The Biochemical journal · 2023Article
- Multi-omics profiling reveals comprehensive microbe-plant-metabolite regulation patterns for medicinal plant Glycyrrhiza uralensis Fisch.Plant biotechnology journal · 2022Article
- SMRT sequencing of full-length transcriptome and gene expression analysis in two chemical types ofPeerJ · 2022Article
- Plastid methylerythritol phosphate pathway participates in the hypersensitive response-related cell death inFrontiers in plant science · 2022Article
- Metabolic Engineering of the Native Monoterpene Pathway in Spearmint for Production of Heterologous Monoterpenes Reveals Complex Metabolism and Pathway Interactions.International journal of molecular sciences · 2020Article
- Metabolomics profiling reveals new aspects of dolichol biosynthesis in Plasmodium falciparum.Scientific reports · 2020Article
- The role of salicylic acid and gibberellin signaling in plant responses to abiotic stress with an emphasis on heavy metals.Plant signaling & behavior · 2020Article
- Transcriptomic profiling reveals MEP pathway contributing to ginsenoside biosynthesis in Panax ginseng.BMC genomics · 2019Article
- Alternative Carbon Sources for Isoprene Emission.Trends in plant science · 2018Review
- A multifunctional oxidosqualene cyclase fromRSC advances · 2018Article
- Dynamics of Monoterpene Formation in Spike Lavender Plants.Metabolites · 2017Article
- SmMYB36, a Novel R2R3-MYB Transcription Factor, Enhances Tanshinone Accumulation and Decreases Phenolic Acid Content in Salvia miltiorrhiza Hairy Roots.Scientific reports · 2017Article
- Polyprenols Are Synthesized by a PlastidialThe Plant cell · 2017Article
- Modeling of Dolichol Mass Spectra Isotopic Envelopes as a Tool to Monitor Isoprenoid Biosynthesis.Plant physiology · 2017Article
- Molecular Farming in Artemisia annua, a Promising Approach to Improve Anti-malarial Drug Production.Frontiers in plant science · 2016Review
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant isoprenoids are derived from two biosynthetic pathways, the cytoplasmic mevalonate (MVA) and the plastidial methylerythritol phosphate (MEP) pathway. In this study their respective contributions toward formation of dolichols in Coluria geoides hairy root culture were estimated using in vivo labeling with (13)C-labeled glucose as a general precursor. NMR and mass spectrometry showed that both the MVA and MEP pathways were the sources of isopentenyl diphosphate incorporated into polyisoprenoid chains. The involvement of the MEP pathway was found to be substantial at the initiation stage of dolichol chain synthesis, but it was virtually nil at the terminal steps; statistically, 6-8 isoprene units within the dolichol molecule (i.e. 40-50% of the total) were derived from the MEP pathway. These results were further verified by incorporation of [5-(2)H]mevalonate or [5,5-(2)H(2)]deoxyxylulose into dolichols as well as by the observed decreased accumulation of dolichols upon treatment with mevinolin or fosmidomycin, selective inhibitors of either pathway. The presented data indicate that the synthesis of dolichols in C. geoides roots involves a continuous exchange of intermediates between the MVA and MEP pathways. According to our model, oligoprenyl diphosphate chains of a length not exceeding 13 isoprene units are synthesized in plastids from isopentenyl diphosphate derived from both the MEP and MVA pathways, and then are completed in the cytoplasm with several units derived solely from the MVA pathway. This study also illustrates an innovative application of mass spectrometry for qualitative and quantitative evaluation of the contribution of individual metabolic pathways to the biosynthesis of natural products.
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